Anti-MHC Class 1 H2 Db抗体[27-11-13] (ab25244)


  • 产品名称
    Anti-MHC Class 1 H2 Db抗体[27-11-13]
    参阅全部 MHC Class 1 H2 Db 一抗
  • 描述
    小鼠单克隆抗体[27-11-13] to MHC Class 1 H2 Db
  • 特异性
    ab25244 recognizes the a3 domain of H2Db class I MHC antigen. The antibody cross-reacts with the a3 domain of H2Ld, H2Dq and H2Lq, but not H2Kd or H2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • 经测试应用
    适用于: Functional Studies, Flow Cyt, IHC-Frmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    C3H.SW splenocytes (Mouse)

  • 表位
    Recognizes an epitope on the a chain of H2Db.
  • 常规说明

    ab25244 can be used for complement-dependent cytoxicity assays.


  • 形式
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Primary antibody说明
    ab25244 can be used for complement-dependent cytoxicity assays.
  • 克隆
  • 克隆编号
  • 同种型
  • 轻链类型
  • 研究领域


Our Abpromise guarantee covers the use of ab25244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Functional Studies Use at an assay dependent concentration.

Complement-dependent cytoxicity assay.

Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.


  • 相关性
    In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • 细胞定位
    Cell Membrane; single pass type I membrane protein
  • 数据库链接
  • 别名
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all


This product has been referenced in:
  • Dower SK  et al. The interaction of monoclonal antibodies with MHC class I antigens on mouse spleen cells. I. Analysis of the mechanism of binding. J Immunol 132:751-8 (1984). Read more (PubMed: 6690617) »

See 1 Publication for this product


Merci pour votre réponse. Je suis désolée que le ab25334 ne convient donc pas. Je vous transmet les détails du code pour tester le ab25244. Veuillez noter que la remise de 100% est applicable sur un anticorps ultérieur, après que nous avons reçu le fee...

Read More

Merci pour votre émail.
Je m'excuse du délais de ma réponse. Effectivement, en discutant avec une collègue, j'ai trouvé que nous avons aussi le clone 34-1-2 dans notre catalogue. Dans la publication il me semblait donner une meilleure valeur pour ...

Read More

Merci de votre intérêt pour ab25244.

J'ai contacté le laboratoire pour avoir plus de détails sur les études fonctionnelles faites avec cet anticorps. Dans l'article ci-dessus les auteurs ont utilisé ce clone pour des études d'inhibition. Read More

Thank you very much for your valuable feedback. We are glad to hear the antibody is working well with your new protocol. We would be very grateful if you could submit an Abreview about this product via the online product datasheet. We always encoura...

Read More