Anti-MGST3抗体[2G10CG7] (ab110309)

概述

  • 产品名称Anti-MGST3抗体[2G10CG7]
    参阅全部 MGST3 一抗
  • 描述
    小鼠单克隆抗体[2G10CG7] to MGST3
  • 经测试应用适用于: ICC/IF, In-Cell ELISA, IPmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    purified enzymes from bovine liver

  • 阳性对照
    • ICC/IF: HDFn cells IP: Human and Rat liver lysates
  • 常规说明

    Product was previously marketed under the MitoSciences sub-brand.

性能

应用

Our Abpromise guarantee covers the use of ab110309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 2 µg/ml.
In-Cell ELISA Use a concentration of 2 µg/ml. (0.2 µg/well)
IP Use at an assay dependent dilution.

靶标

  • 功能Also functions as a glutathione peroxidase.
  • 组织特异性Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes.
  • 序列相似性Belongs to the MAPEG family.
  • 细胞定位Endoplasmic reticulum membrane. Microsome membrane. Microsome membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Mgst3 antibody
    • MGST3_HUMAN antibody
    • Microsomal glutathione S transferase 3 antibody
    • Microsomal glutathione S-transferase 3 antibody
    • Microsomal GST-3 antibody
    • Microsomal GST-III antibody
    • Microsomal GST3 antibody
    • Microsomal GSTIII antibody
    see all

Anti-MGST3 antibody [2G10CG7] 图像

  • Immunocytochemistry image of ab110309-stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110309 at 2 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates primarily in the mitochondria.
  • Using MitoSciences' standard immunoprecipitation protocol, ab110309 pulls down the 15kDa MGST3 protein from human and rat liver samples. HepG2 lysate and mouse liver lysate yielded smaller amounts. This identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

Anti-MGST3 antibody [2G10CG7] (ab110309)参考文献

ab110309 has not yet been referenced specifically in any publications.

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