使用敲除细胞株进行验证RabMAb

Anti-MGMT抗体[EPR4397] (ab108630)

概述

  • 产品名称Anti-MGMT抗体[EPR4397]
    参阅全部 MGMT 一抗
  • 描述
    兔单克隆抗体[EPR4397] to MGMT
  • 经测试应用适用于: WB, IHC-Pmore details
    不适用于: Flow Cyt,ICC or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    A synthetic peptide corresponding to residues in Human MGMT.

  • 阳性对照
    • WB: MCF7, Jurkat, HeLa, and HT29 cell lysates IHC-P: Hman clear cell carcinoma and spleen tissues
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108630 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 22 kDa).
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • 应用说明Is unsuitable for Flow Cyt,ICC or IP.
  • 靶标

    • 功能Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
    • 序列相似性Belongs to the MGMT family.
    • 细胞定位Nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 6 O methylguanine DNA methyltransferase antibody
      • 6-O-methylguanine-DNA methyltransferase antibody
      • Agat antibody
      • AGT antibody
      • AI267024 antibody
      • EC 2.1.1.63 antibody
      • Methylated DNA protein cysteine methyltransferase antibody
      • Methylated-DNA--protein-cysteine methyltransferase antibody
      • Methylguanine DNA methyltransferase antibody
      • MGC107020 antibody
      • MGMT antibody
      • MGMT_HUMAN antibody
      • O 6 methylguanine DNA alkyltransferase antibody
      • O 6 methylguanine DNA methyltransferase antibody
      • O-6-methylguanine-DNA methyltransferase antibody
      • O-6-methylguanine-DNA-alkyltransferase antibody
      see all

    Anti-MGMT antibody [EPR4397] 图像



    • Predicted band size : 22 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MGMT knockout HAP1 cell lysate (20 µg)
      Lane 3: MCF7 cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab108630 observed at 22 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab108630 was shown to specifically react with MGMT when MGMT knockout samples were used. Wild-type and MGMT knockout samples were subjected to SDS-PAGE. ab108630 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-MGMT antibody [EPR4397] (ab108630) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : Jurkat ell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 22 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human clear cell carcinoma tissue by Immunohistochemistry.
    • ab108630, at a 1/250 dilution, staining MGMT in paraffin embedded Human spleen tissue by Immunohistochemistry.

    Anti-MGMT antibody [EPR4397] (ab108630)参考文献

    ab108630 has not yet been referenced specifically in any publications.

    Product Wall

    Leider ist es so, wie ich gestern schon vermutete: Wir haben die Antikörper- Konzentration in dem Zellkultur- Überstand leider nicht bestimmt.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"