Anti-MGAT5抗体[3E9] (ab87977)
Key features and details
- Mouse monoclonal [3E9] to MGAT5
- Suitable for: WB, Flow Cyt
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
概述
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产品名称
Anti-MGAT5抗体[3E9]
参阅全部 MGAT5 一抗 -
描述
小鼠单克隆抗体[3E9] to MGAT5 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cytmore details -
种属反应性
与反应: Human, Recombinant fragment
预测可用于: Mouse, Rat, Hamster -
免疫原
Recombinant fragment: LAEPGQSCKQ VCQESQLICE PSFFQHLNKD KDMLKYKVTC QSSELAKDIL VPSFDPKNK HCVFQGDLLL FSCAGAHPRH QRVCPCRDFI KGQVALCKD, corresponding to amino acids 642 - 739 of Human MGAT5 (NP_002401) with a proprietary tag
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阳性对照
- Jurkat whole cell lysate (ab7899)
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常规说明
This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
纯化说明
Purified from TCS. -
克隆
单克隆 -
克隆编号
3E9 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab87977于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
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Flow Cyt |
Use at an assay dependent concentration.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
说明 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 85 kDa. |
Flow Cyt
Use at an assay dependent concentration. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
靶标
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功能
Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides. -
通路
Protein modification; protein glycosylation. -
序列相似性
Belongs to the glycosyltransferase 18 family. -
细胞定位
Golgi apparatus membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4249 Human
- Entrez Gene: 107895 Mouse
- Entrez Gene: 65271 Rat
- Omim: 601774 Human
- SwissProt: Q09328 Human
- SwissProt: Q8R4G6 Mouse
- SwissProt: Q08834 Rat
- Unigene: 651869 Human
see all -
别名
- 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A antibody
- 6-N-acetylglucosaminyltransferase antibody
- Alpha 1,3(6) mannosylglycoprotein antibody
see all
图片
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Anti-MGAT5 antibody [3E9] (ab87977) at 1/2000 dilution + Human RWPE prostate cells at 20 µg
Secondary
Donkey anti-mouse polyclonal HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Additional bands at: 55 kDa (possible non-specific binding), 95 kDa (possible post-translational modification)
Exposure time: 40 secondsBlocking was via 5% milk in PBST incubated for 1 hour at 22°C
This image was generated using the ascites version of the product.
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Overlay histogram showing Jurkat cells stained with ab87977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87977, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This image was generated using the ascites version of the product.
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Anti-MGAT5 antibody [3E9] (ab87977) at 1 µg/ml + recombinant protein fragment with a proprietary tag (the immunogen) at 0.1 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size: 85 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?The recombinant protein used as the immunogen has a predicted size of 37 kDa.
This image was generated using the ascites version of the product.
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Anti-MGAT5 antibody [3E9] (ab87977) at 1 µg/ml + jurkat cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 85 kDaThis image was generated using the ascites version of the product.
数据表及文件
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Datasheet download
文献 (4)
ab87977 被引用在 4 文献中.
- Cheng CC et al. Overexpression of N-acetylglucosaminyltransferase V promotes human parotid gland acinar cell immortalization via the epidermal receptor activation. J Cell Physiol 237:1780-1789 (2022). PubMed: 34806177
- Cong X et al. Silencing GnT-V reduces oxaliplatin chemosensitivity in human colorectal cancer cells through N-glycan alteration of organic cation transporter member 2. Exp Ther Med 21:128 (2021). PubMed: 33376510
- Yan G et al. Decreased miR-124-3p promoted breast cancer proliferation and metastasis by targeting MGAT5. Am J Cancer Res 9:585-596 (2019). PubMed: 30949412
- Wang G et al. Loss of Barx1 promotes hepatocellular carcinoma metastasis through up-regulating MGAT5 and MMP9 expression and indicates poor prognosis. Oncotarget 8:71867-71880 (2017). PubMed: 29069753