Anti-MGAT5抗体(ab68595)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MGAT5
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Sheep, Horse, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human MGAT5.

    (Peptide available as ab68594.)

  • 阳性对照
    • This antibody gave a positive signal in the following Whole Cell Lysates: TE 671, Jurkat, Jurkat - Staurosporine Treated, MEF1 (data not shown) and HeLa - Hydroxyurea Treated (48hr, 2uM) (data not shown).

性能

应用

Our Abpromise guarantee covers the use of ab68595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 84 kDa).Can be blocked with Human MGAT5 peptide (ab68594).

靶标

  • 功能
    Catalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
  • 通路
    Protein modification; protein glycosylation.
  • 序列相似性
    Belongs to the glycosyltransferase 18 family.
  • 细胞定位
    Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 6-mannosylglycoprotein 6-beta-N-acetylglucosaminyltransferase A antibody
    • 6-N-acetylglucosaminyltransferase antibody
    • Alpha 1,3(6) mannosylglycoprotein antibody
    • Alpha 1,6 mannosylglycoprotein 6 beta N acetylglucosaminyltransferase antibody
    • Alpha mannoside beta 1,6 N acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha-mannoside beta-1 antibody
    • Beta 1,6 N acetyl glucosaminyltransferase antibody
    • GGNT5 antibody
    • GlcNAc T V antibody
    • GlcNAc-T V antibody
    • GNT V antibody
    • GNT VA antibody
    • GNT-V antibody
    • GNTV antibody
    • GNTVA antibody
    • Mannoside acetylglucosaminyltransferase 5 antibody
    • Mannosyl (alpha 1,6) glycoprotein beta 1,6 N acetyl glucosaminyltransferase antibody
    • Mgat5 antibody
    • MGT5A_HUMAN antibody
    • N acetylglucosaminyl transferase V antibody
    • N acetylglucosaminyltransferase V mannosyl (alpha 1,6) glycoprotein antibody
    • N-acetylglucosaminyl-transferase V antibody
    see all

图片

  • All lanes : Anti-MGAT5 antibody (ab68595) at 1 µg/ml

    Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 84 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa,70 kDa. We are unsure as to the identity of these extra bands.

实验方案

文献

ab68595 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
25 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

提交于 Nov 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Mar 28 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

提交于 Jan 24 2017

Thank you for contacting us. MEF1 cells were used to test for mouse reactivity in WB. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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