Anti-MGAT2抗体(ab62526)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to MGAT2
  • 宿主
    Rabbit
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Monkey, Zebrafish, Cynomolgus monkey, Macaque monkey
  • 免疫原

    A region within synthetic peptide: SIQLYPNEFP GSDPRDCPRD LPKNAALKLG CINAEYPDSF GHYREAKFSQ, corresponding to internal sequence amino acids 180-229 of Human MGAT2

  • 阳性对照
    • Jurkat cell lysate. Human kidney tissue.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab62526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

靶标

  • 相关性
    MGAT2 is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in its gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain.
  • 细胞定位
    Golgi Apparatus membrane; type II membrane protein
  • 数据库链接
  • 别名
    • Alpha 1,6 mannosyl glycoprotein 2 beta N acetylglucosaminyltransferase antibody
    • Beta 1,2 N acetylglucosaminyltransferase II antibody
    • CDG2A antibody
    • CDGS2 antibody
    • GlcNAc T II antibody
    • GLCNACTII antibody
    • GNT II antibody
    • GNT2 antibody
    • Mannoside acetylglucosaminyltransferase 2 antibody
    • N glycosyl oligosaccharide glycoprotein N acetylglucosaminyltransferase II antibody
    • UDP N acetylglucosamine:alpha 6 D mannoside beta 1,2 N acetylglucosaminyltransferase II antibody
    see all

图片

  • Anti-MGAT2 antibody (ab62526) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size: 52 kDa
    Observed band size: 52 kDa



    Gel concentration: 12%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling MGAT2 with ab62526 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnification: 400X.

文献

ab62526 has not yet been referenced specifically in any publications.

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - other (Neuroblastoma)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
20 µg
Specification
Neuroblastoma
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Username

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提交于 Mar 02 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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