概述

  • 产品名称
  • 描述
    山羊多克隆抗体to MGAT1
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat, Rabbit
  • 免疫原

    Synthetic peptide:

    C-QVEKVRTNDR

    , corresponding to internal sequence amino acids 369-378 of Human MGAT1 (NP_002397.2).

  • 阳性对照
    • MGAT1 transfected HEK293 lysate

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab109552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

靶标

  • 功能
    Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
  • 组织特异性
    Appears to be present in all tissues.
  • 通路
    Protein modification; protein glycosylation.
  • 序列相似性
    Belongs to the glycosyltransferase 13 family.
  • 细胞定位
    Golgi apparatus membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
    • GGNT1 antibody
    • GLCNAC TI antibody
    • GlcNAc-T I antibody
    • GLCT1 antibody
    • GLYT1 antibody
    • GNT 1 antibody
    • GNT-I antibody
    • Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase antibody
    • MGAT antibody
    • MGAT1 antibody
    • MGAT1_HUMAN antibody
    • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I antibody
    see all

图片

  • All lanes : Anti-MGAT1 antibody (ab109552) at 1 µg/ml

    Lane 1 : HEK293 lysate in RIPA buffer, overexpressing Human MGAT1 with C terminal MYC tag
    Lane 2 : Mock transfected HEK293 lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

实验方案

文献

ab109552 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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