, corresponding to internal sequence amino acids 369-378 of Human MGAT1 (NP_002397.2).
- MGAT1 transfected HEK293 lysate
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).
Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
Appears to be present in all tissues.
Protein modification; protein glycosylation.
Belongs to the glycosyltransferase 13 family.
Golgi apparatus membrane.
Information by UniProt
- 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
- Alpha-1 antibody
- Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
Western blot - MGAT1 antibody (ab109552)
All lanes :
Anti-MGAT1 antibody (ab109552) at 1 µg/mlLane 1 :
HEK293 lysate in RIPA buffer, overexpressing Human MGAT1 with C terminal MYC tag Lane 2 :
Mock transfected HEK293 lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL techniquePredicted band size :
50 kDaObserved band size :
55 kDa (why is the actual band size different from the predicted?
)Exposure time :
has not yet been referenced specifically in any publications.
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