概述

  • 产品名称Anti-MFF抗体
    参阅全部 MFF 一抗
  • 描述
    兔多克隆抗体to MFF
  • 经测试应用适用于: ICC/IF, WBmore details
  • 种属反应性
    与反应: Rat, Human, Zebrafish
    预测可用于: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • 免疫原

    Synthetic peptide corresponding to a region within C terminal amino acids 287-336 (LTVVDAASLR RQIIKLNRRL QLLEEENKER AKREMVMYSI TVAFWLLNSW) of Human MFF (NP_064579).

  • 阳性对照
    • Fetal muscle lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549

性能

应用

Our Abpromise guarantee covers the use of ab81127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

Anti-MFF antibody 图像

  • Anti-MFF antibody (ab81127) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
  • All lanes : Anti-MFF antibody (ab81127) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • ab81127 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81127 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MFF antibody (ab81127)参考文献

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abreviews
Application Western blot
Sample Mouse Cell lysate - whole cell (p38 mouse melanoma cell line)
Gel Running Conditions Reduced Denaturing
Loading amount 40 µg
Treatment Mff KD siRNA
Specification p38 mouse melanoma cell line
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Carmen Adriaens

Verified customer

提交于 Aug 07 2015


The immunogens differ, though both are closer to the C terminus than the N terminus. The immunogen for ab81127 is derived from within (but is not identical to) the range given on the datasheet, amino acids 287-336 of human MFF. The immunogen for a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (Neurones primaires corticaux)
Loading amount 1.2e+006 cells
Specification Neurones primaires corticaux
Gel Running Conditions Reduced Denaturing (gel 15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mrs. Marlene Daloyau

Verified customer

提交于 Oct 16 2012

Code de remise pour le test de ab74257: *******
Code de remise pour le test de ab81127 : ****** (ci-joint l'alignement de la région aa 30-100 avec la protéine de rat)
Code de remise pour le test de ab101908 : *******

Date...

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Thank you for contacting us.

Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen secti...

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