Synthetic peptide from the N terminal region of human METTL7A, conjugated to KLH.
293 cell lysates transiently transfected with the METTL7A gene. Human hepatocarcinoma tissue.
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Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein A purified
ab62969 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 28 kDa).
1/10 - 1/50.
Belongs to the methyltransferase superfamily.
Lipid droplet. Endoplasmic reticulum. Membrane.
Information by UniProt
AAM B antibody
Western blot - Anti-METTL7A antibody (ab62969)
All lanes :
Anti-METTL7A antibody (ab62969) at 1/50 dilution
Lane 1 :
293 cell lysates nontransfected
Lane 2 :
293 cell lysates transiently transfected with the METTL7A gene
Lysates/proteins at 2 µg per lane.
Predicted band size:
Observed band size:
25 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL7A antibody (ab62969)
ab62969, at 1/10 dilution, staining METTL7A in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by immunohistochemistry. ab62969 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
has not yet been referenced specifically in any publications.
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