概述

  • 产品名称
  • 描述
    兔多克隆抗体to METTL3
  • 经测试应用
    适用于: ELISA, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human METTL3 (internal sequence).
    Sequence:

    AASDVDLEIESLLNQQSTKEQQSKKVSQEILELLNTTTAKEQSIVEKFRS

  • 阳性对照
    • Raji cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab49253 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

WB Use a concentration of 5 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    N6-methyltransferase that methylates adenosine residues of some mRNAs. N6-methyladenosine (m6A), which is present at internal sites of some mRNAs, may play a role in the efficiency of mRNA splicing, transport or translation.
  • 组织特异性
    Widely expressed at low level. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocytes.
  • 序列相似性
    Belongs to the MT-A70-like family.
  • 翻译后修饰
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位
    Nucleus speckle. Colocalizes with speckles in interphase nuclei. Suggesting that it may be associated with nuclear pre-mRNA splicing components.
  • Information by UniProt
  • 数据库链接
  • 别名
    • adoMet-binding subunit of the human mRNA (N6-adenosine)-methyltransferase antibody
    • IME4 antibody
    • M6A antibody
    • Methyltransferase like protein 3 antibody
    • Methyltransferase-like protein 3 antibody
    • METTL3 antibody
    • mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase antibody
    • mRNA m(6)A methyltransferase antibody
    • MT-A70 antibody
    • MTA70 antibody
    • MTA70_HUMAN antibody
    • N6 adenosine methyltransferase 70 kDa subunit antibody
    • N6-adenosine-methyltransferase 70 kDa subunit antibody
    see all

Anti-METTL3 antibody 图像

  • Anti-METTL3 antibody (ab49253) at 5 µg/ml + Raji cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG 1/50,000 - 1/100,000

    Predicted band size : 64 kDa
    Observed band size : 64 kDa

实验方案

Anti-METTL3 antibody (ab49253)参考文献

This product has been referenced in:

See 1 Publication for this product

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