Recombinant fragment, corresponding to amino acids 3-143 of Human METTL21D (Q9H867-1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 - 4 µg/ml.
Recommend PFA Fixation and Triton X-100 treatment
||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (retrieval buffer pH 6).
Belongs to the methyltransferase superfamily. METTL21 family.
Information by UniProt
- C14orf138 antibody
- Methyltransferase like 21D antibody
- Methyltransferase-like protein 21D antibody
Immunocytochemistry/ Immunofluorescence - Anti-METTL21D antibody (ab121127)
Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121127 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL21D antibody (ab121127)
ab121127 at 1/125 dilution staining METTL21D in Paraffin-emebedded Human breast tissue by Immunohistochemistry.
has not yet been referenced specifically in any publications.
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