概述
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产品名称Anti-METTL11A抗体
参阅全部 METTL11A 一抗 -
描述兔多克隆抗体to METTL11A
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宿主Rabbit
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Human
预测可用于: Mouse, Rat, Horse, Cow, Dog, Macaque monkey
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免疫原
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human METTL11A.
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阳性对照
- This antibody gave a positive signal in the following whole cell lysates: HeLa; HeLa Nuclear; HEK293; U20S; Jurkat.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab102664 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 25 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
靶标
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功能Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala, Pro or Ser residue in the [Ala/Pro/Ser]-Pro-Lys motif. Mediates the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation.
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序列相似性Belongs to the methyltransferase superfamily. NTM1 family.
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细胞定位Nucleus. Predominantly nuclear.
- Information by UniProt
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数据库链接
- Entrez Gene: 28989 Human
- Entrez Gene: 66617 Mouse
- Entrez Gene: 362103 Rat
- Omim: 613560 Human
- SwissProt: Q9BV86 Human
- SwissProt: Q8R2U4 Mouse
- SwissProt: Q5BJX0 Rat
- Unigene: 522433 Human
see all -
别名
- AD 003 antibody
- Alpha N-terminal protein methyltransferase 1A antibody
- C9orf32 antibody
see all
图片
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Western blot - Anti-METTL11A antibody (ab102664)All lanes : Anti-METTL11A antibody (ab102664) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate
Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 26 kDa (why is the actual band size different from the predicted?)
Additional bands at: 150 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-METTL11A antibody (ab102664)ICC/IF image of ab102664 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab102664 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in HeLa cells, 100% Methanol fixed for 5minutes at 5ug/ml
文献
ab102664 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"