Anti-Methylmalonyl Coenzyme A mutase抗体(ab67869)


  • 产品名称
    Anti-Methylmalonyl Coenzyme A mutase抗体
    参阅全部 Methylmalonyl Coenzyme A mutase 一抗
  • 描述
    小鼠多克隆抗体to Methylmalonyl Coenzyme A mutase
  • 宿主
  • 经测试应用
    适用于: ICC/IF, WB, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
  • 免疫原

    Full length Methylmalonyl Coenzyme A mutase protein (Human)

  • 阳性对照
    • Methylmalonyl Coenzyme A mutase transfected 293T cell lysate



Our Abpromise guarantee covers the use of ab67869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P Use at an assay dependent dilution. PubMed: 19699272


  • 功能
    Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.
  • 疾病相关
    Defects in MUT are the cause of methylmalonic aciduria type mut (MMAM) [MIM:251000]. MMAM is an often fatal disorder of organic acid metabolism. Common clinical features include lethargy, vomiting, failure to thrive, hypotonia, neurological deficit and early death. Two forms of the disease are distinguished by the presence (mut-) or absence (mut0) of residual enzyme activity. Mut0 patients have more severe neurological manifestations of the disease than do MUT- patients. MMAM is unresponsive to vitamin B12 therapy.
  • 序列相似性
    Belongs to the methylmalonyl-CoA mutase family.
    Contains 1 B12-binding domain.
  • 细胞定位
    Mitochondrion matrix.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MCM antibody
    • Methylmalonyl CoA isomerase antibody
    • Methylmalonyl CoA mutase mitochondrial antibody
    • Methylmalonyl Coenzyme A mutase antibody
    • Methylmalonyl-CoA isomerase antibody
    • Methylmalonyl-CoA mutase antibody
    • mitochondrial antibody
    • Mut antibody
    • MUTA_HUMAN antibody
    see all


  • Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution + human liver tissue lysate at 25 µg

    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution

    Lane 1 : Methylmalonyl Coenzyme A mutase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size: 83 kDa
    Observed band size: 83 kDa

  • ICC/IF image of ab67869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67869, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/2000 dilution

    Lane 1 : Euglena whole cell lysate with Milk and 0.05% Tween20
    Lane 2 : Euglena (RNAi gene silencing Methylmalonyl Coenzyme A mutase) whole cell lysate, with Milk and 0.05% Tween20

    Lysates/proteins at 15 µg per lane.

    All lanes : Goat Anti-mouse IgG(H+L) HRP conjugated at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size: 83 kDa

    Exposure time: 10 minutes

    See Abreview


This product has been referenced in:
  • Ruppert T  et al. Molecular and biochemical alterations in tubular epithelial cells of patients with isolated methylmalonic aciduria. Hum Mol Genet 24:7049-59 (2015). Read more (PubMed: 26420839) »
  • Ballhausen D  et al. Evidence for catabolic pathway of propionate metabolism in CNS: expression pattern of methylmalonyl-CoA mutase and propionyl-CoA carboxylase alpha-subunit in developing and adult rat brain. Neuroscience 164:578-87 (2009). WB, IHC-P ; Rat . Read more (PubMed: 19699272) »

See all 2 Publications for this product


Western blot
Euglena Cell lysate - whole cell (Euglena gracilis SM-ZK)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Gene silencing
Euglena gracilis SM-ZK
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

昌美 中澤

Verified customer

提交于 Oct 31 2017

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for ICC...

Read More