Anti-Methylated Lysine (di methyl ,mono methyl )抗体(ab23366)
Key features and details
- Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl )
- Suitable for: IHC-P, WB, ELISA
- Reacts with: Species independent
- Isotype: IgG
概述
-
产品名称
Anti-Methylated Lysine (di methyl ,mono methyl )抗体
参阅全部 Methylated Lysine 一抗 -
描述
兔多克隆抗体to Methylated Lysine (di methyl ,mono methyl ) -
宿主
Rabbit -
特异性
Recognize proteins methylated on lysine residues (mono- & di-methyl lysine). This antibody was affinity purified with mono-methyl lysine and di-methyl lysine, and may also react with tri-methyl lysine. Does not cross-react with acetylated protein. -
经测试应用
适用于: IHC-P, WB, ELISAmore details -
种属反应性
与反应: Species independent -
免疫原
Methylated Lysine conjugated to KLH.
-
阳性对照
- HCL (0.1N) extracted and acetone precipited histone fraction
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 6.00
Constituents: PBS, 50% Glycerol -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Affinity purified using N-methyl (epsilon amino group)lysine on Agarose as affinity matrix. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab23366于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P | (3) |
Use at an assay dependent concentration.
|
WB | (2) |
Use a concentration of 0.5 - 1 µg/ml. Do not use milk for any of the buffers as complex milk proteins may contain competitive methylated proteins. Use BSA instead. ab23366 incubation time 2h RT. If no or weak signal is obtained overnight at 4deg might help.
|
ELISA |
Use at an assay dependent concentration.
|
说明 |
---|
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.5 - 1 µg/ml. Do not use milk for any of the buffers as complex milk proteins may contain competitive methylated proteins. Use BSA instead. ab23366 incubation time 2h RT. If no or weak signal is obtained overnight at 4deg might help. |
ELISA
Use at an assay dependent concentration. |
靶标
-
相关性
Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains. -
别名
- Dimethylysine antibody
- Methyl lysine antibody
- N epsilon dimethyl lysine antibody
see all
图片
-
Lane 1 : Anti-Methylated Lysine (tri methyl) antibody (does not cross react with mono, or di methyl lysine residues)
Lane 2 : Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)
All lanes : Yeast extract -
ab23366 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed a heat mediated antigen retrieval step was performed in citrate buffer. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
-
ab23366 staining formalin fixed paraffin-embedded rat kidney tissue sections. The section was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 5% serum for 30 minutes at 20°C, prior to incubation with the primary antibody (diluted 1/200 in PBS + 5% serum) for 45 mintes at 20°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.
数据表及文件
-
SDS download
-
Datasheet download
文献 (45)
ab23366 被引用在 45 文献中.
- Chen S et al. Loss of SPTBN1 Suppresses Autophagy Via SETD7-mediated YAP Methylation in Hepatocellular Carcinoma Initiation and Development. Cell Mol Gastroenterol Hepatol 13:949-973.e7 (2022). PubMed: 34737104
- Luo H et al. MAT2A facilitates PDCD6 methylation and promotes cell growth under glucose deprivation in cervical cancer. Cell Death Discov 8:176 (2022). PubMed: 35396512
- Hou Y et al. Epithelial SMYD5 Exaggerates IBD by Down-regulating Mitochondrial Functions via Post-Translational Control of PGC-1α Stability. Cell Mol Gastroenterol Hepatol 14:375-403 (2022). PubMed: 35643234
- Zhang Y et al. Unique SMYD5 Structure Revealed by AlphaFold Correlates with Its Functional Divergence. Biomolecules 12:N/A (2022). PubMed: 35740908
- Sullivan MR et al. Methionine synthase is essential for cancer cell proliferation in physiological folate environments. Nat Metab 3:1500-1511 (2021). PubMed: 34799701