Methylated DNA Quantification试剂盒(Fluorometric) (ab117129)

概述

  • 产品名称Methylated DNA Quantification试剂盒(Fluorometric)
    参阅全部 5-methylcytosine 试剂盒
  • 样品类型
    Saliva, Urine, Plasma, Tissue, Adherent cells, Suspension cells
  • 检测类型Quantitative
  • 灵敏度
    >= 50 pg/well
  • 检测时间
    4h 00m
  • 种属反应性
    与反应: Species independent
  • 产品概述

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

    Abcam's Methylated DNA Quantification Kit (Fluorometric) (ab117129) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

    It can be paired to use with Hydroxymethylated DNA Quantification Kit (Fluorometric) (ab117131), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab117129 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Methylated DNA Quantification Kit (Fluorometric) 图像

    • Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117129. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117129.

    实验方案

    Methylated DNA Quantification Kit (Fluorometric) (ab117129)参考文献

    ab117129 has not yet been referenced specifically in any publications.

    Product Wall


    Having reviewed this case with the lab, I would like to offer some suggestions to help optimize the results from ab117129.

    It appears that the signal may be saturated either due to

    1) insufficient washing or
    2) too much ti...

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    Thank you for your enquiry and interest regarding ab117129.
    I can confirm that the TE buffer specified in the protocol is constituted with 10 mM Tris-HCl pH 7.5-8 and 1 mM EDTA.
    If you need any further assistance in the future, please do not ...

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    Thank you for contacting Abcam.


    Yes, the assay detect 5-mC on both single and double stranded DNA with the nearly same sensitivity. The standard is double stranded DNA.



    Please let me know if there is anything else I can...

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    Thank you for contacting us.

    Your credit note ID is ******.

    I am sorry that this kit did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in one of...

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    Thank you very much for your patience.

    We have now received the stimulation graph from the laboratory. Please find it in the attachment. Based on that graph, I am happy to confirm that stimulation at 550nm is absolutely fine, as well as emiss...

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    I would like to inform you that we are still investigating the problem you experienced with the kits. The lab has already provided the following information: "The reading can be carried out at 530/590 nm +/- 10 generally. Thus, emission at 59...

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