Methylated DNA Immunoprecipitation (MeDIP) ChIP试剂盒(ab117135)

概述

  • 产品名称Methylated DNA Immunoprecipitation (MeDIP) ChIP试剂盒
    参阅全部 5-methylcytosine 试剂盒
  • 样品类型
    Adherent cells, Suspension cells
  • 检测时间
    4h 00m
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 产品概述

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

    Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

  • 经测试应用适用于: Functional Studiesmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab117135 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    实验方案

    Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135)参考文献

    ab117135 has not yet been referenced specifically in any publications.

    Product Wall

    I apologize again for the incorrect information given originally.

    For tissues we recommend, ab117136.
    In comparison, kit ab117135 would be used when starting with cells. When using a DNA sample, please use ab117133 instead.

    I ...

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    Both of these kits (ab117133 and ab117135) will immunoprecipitate methylated DNA. The ab117133 Kit uses purified DNA as starting materials while the ab117135 Kit uses cells as starting materials. I can confirm that the immunoprecipitated products are s...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"