Anti-Methionine Sulfoxide Reductase A抗体(ab16803)

概述

  • 产品名称
    Anti-Methionine Sulfoxide Reductase A抗体
    参阅全部 Methionine Sulfoxide Reductase A 一抗
  • 描述
    兔多克隆抗体to Methionine Sulfoxide Reductase A
  • 经测试应用
    适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
    预测可用于: Macaque monkey
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • Mouse kidney extract.

性能

应用

Our Abpromise guarantee covers the use of ab16803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能
    Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
  • 组织特异性
    Ubiquitous. Highest expression in adult kidney and cerebellum, followed by liver, heart ventricles, bone marrow and hippocampus.
  • 序列相似性
    Belongs to the MsrA Met sulfoxide reductase family.
  • 细胞定位
    Cytoplasm; Cytoplasm. Nucleus and Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosolic methionine S sulfoxide reductase antibody
    • Methionine sulphoxide reductase A antibody
    • Mitochondrial peptide methionine sulfoxide reductase antibody
    • MSR A antibody
    • msrA antibody
    • MSRA_HUMAN antibody
    • peptide met (O) reductase antibody
    • Peptide Met(O) reductase antibody
    • Peptide methionine (S) S oxide reductase antibody
    • Peptide-methionine (S)-S-oxide reductase antibody
    • PMSR antibody
    • Protein methionine S oxide reductase antibody
    • Protein-methionine-S-oxide reductase antibody
    see all

图片

  • All lanes : Anti-Methionine Sulfoxide Reductase A antibody (ab16803)

    Lane 1 : Mouse Kidney
    Lane 2 : Rat Kidney


    Predicted band size : 26 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)

    Western blot using ab16803 at 1/2000.

    Lane 1: Mouse kidney
    Lane 2: Rat kidney

  • ab16803 (1µg/ml) staining Methionine Sulfoxide Reductase A in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

文献

This product has been referenced in:
  • Binolfi A  et al. Intracellular repair of oxidation-damaged a-synuclein fails to target C-terminal modification sites. Nat Commun 7:10251 (2016). WB . Read more (PubMed: 26807843) »
  • Ghosh S  et al. Proteometabolomic Study of Compatible Interaction in Tomato Fruit Challenged with Sclerotinia rolfsii Illustrates Novel Protein Network during Disease Progression. Front Plant Sci 7:1034 (2016). WB . Read more (PubMed: 27507973) »

See all 7 Publications for this product

客户评价及客户问答

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The product code of the antibody that was used in the reference listed on the ab16803 datasheet was, as far as we know, ab16803. I believe this information,which is not in the text of the paper, was given to us b...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cow Recombinant protein (None)
Loading amount
0.15 µg
Specification
None
Gel Running Conditions
Reduced Denaturing (4-20% Gradient Pre-cast Gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jul 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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