Anti-Methionine Sulfoxide Reductase A抗体(ab16803)

概述

  • 产品名称Anti-Methionine Sulfoxide Reductase A抗体
    参阅全部 Methionine Sulfoxide Reductase A 一抗
  • 描述
    兔多克隆抗体to Methionine Sulfoxide Reductase A
  • 经测试应用适用于: ELISA, WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
    预测可用于: Macaque Monkey
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • Mouse kidney extract.

性能

应用

Our Abpromise guarantee covers the use of ab16803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 1 µg/ml.

靶标

  • 功能Has an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
  • 组织特异性Ubiquitous. Highest expression in adult kidney and cerebellum, followed by liver, heart ventricles, bone marrow and hippocampus.
  • 序列相似性Belongs to the MsrA Met sulfoxide reductase family.
  • 细胞定位Cytoplasm; Cytoplasm. Nucleus and Mitochondrion.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cytosolic methionine S sulfoxide reductase antibody
    • Methionine sulphoxide reductase A antibody
    • Mitochondrial peptide methionine sulfoxide reductase antibody
    • MSR A antibody
    • msrA antibody
    • MSRA_HUMAN antibody
    • peptide met (O) reductase antibody
    • Peptide Met(O) reductase antibody
    • Peptide methionine (S) S oxide reductase antibody
    • Peptide-methionine (S)-S-oxide reductase antibody
    • PMSR antibody
    • Protein methionine S oxide reductase antibody
    • Protein-methionine-S-oxide reductase antibody
    see all

Anti-Methionine Sulfoxide Reductase A antibody 图像

  • All lanes : Anti-Methionine Sulfoxide Reductase A antibody (ab16803)

    Lane 1 : Mouse Kidney
    Lane 2 : Rat Kidney


    Predicted band size : 26 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • ab16803 (1µg/ml) staining Methionine Sulfoxide Reductase A in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Anti-Methionine Sulfoxide Reductase A antibody (ab16803) at 1/2000 dilution + Methionine Sulfoxide Reductase A protein (Active) (ab82728) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Anti-Methionine Sulfoxide Reductase A antibody (ab16803)参考文献

This product has been referenced in:
  • Zhang C  et al. Anoxia, acidosis, and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells. J Cell Biochem 112:98-106 (2011). WB ; Mouse . Read more (PubMed: 20872796) »
  • Brennan LA  et al. TXNL6 is a novel oxidative stress-induced reducing system for methionine sulfoxide reductase a repair of a-crystallin and cytochrome C in the eye lens. PLoS One 5:e15421 (2010). WB ; Human . Read more (PubMed: 21079812) »

See all 3 Publications for this product

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The product code of the antibody that was used in the reference listed on the ab16803 datasheet was, as far as we know, ab16803. I believe this information,which is not in the text of the paper, was given to us b...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Recombinant protein (None)
Loading amount 0.15 µg
Specification None
Gel Running Conditions Reduced Denaturing (4-20% Gradient Pre-cast Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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提交于 Jul 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"