重组
Anti-Methionine Aminopeptidase 2抗体[EPR6886(B)] (ab124953)
- 数据表
- 具体的参考文献 (1)
- 实验方案
概述
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产品名称Anti-Methionine Aminopeptidase 2抗体[EPR6886(B)]
参阅全部 Methionine Aminopeptidase 2 一抗 -
描述兔单克隆抗体[EPR6886(B)] to Methionine Aminopeptidase 2
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宿主Rabbit
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经测试应用适用于: WB, Flow Cyt, IHC-Frmore details
不适用于: ICC/IF,IHC-P or IP -
种属反应性与反应: Mouse, Rat, Human
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免疫原
Synthetic peptide, corresponding to residues in Human Methionine Aminopeptidase 2.
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阳性对照
- HepG2, 293T, HeLa, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Permeabilized 293T cells.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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纯度Tissue culture supernatant
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克隆单克隆
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克隆编号EPR6886(B)
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同种型IgG
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研究领域
相关产品
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab124953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa). | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| IHC-Fr | Use at an assay dependent concentration. |
应用说明
Is unsuitable for ICC/IF,IHC-P or IP.
靶标
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功能Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. -
序列相似性Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily.
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翻译后修饰Contains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding.
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细胞定位Cytoplasm. About 30% of expressed METAP2 associates with polysomes.
- Information by UniProt
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数据库链接
- Entrez Gene: 10988 Human
- Entrez Gene: 56307 Mouse
- Entrez Gene: 64370 Rat
- Omim: 601870 Human
- SwissProt: P50579 Human
- SwissProt: O08663 Mouse
- SwissProt: P38062 Rat
- Unigene: 444986 Human
see all -
别名
- A930035J23Rik antibody
- AI047573 antibody
- AL024412 antibody
see all
图片
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![Western blot - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953)](http://www.abcam.cn/ps/datasheet/images/124/ab124953/Methionine-Aminopeptidase-2-Primary-antibodies-ab124953-1.jpg)
Western blot - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953)All lanes : Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : 293T cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : C6 cell lysates
Lane 5 : RAW 264.7 cell lysates
Lane 6 : PC12 cell lysates
Lane 7 : NIH 3T3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 67 kDa (why is the actual band size different from the predicted?) -
![Flow Cytometry - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953)](http://www.abcam.cn/ps/datasheet/images/124/ab124953/Methionine-Aminopeptidase-2-Primary-antibodies-ab124953-3.jpg)
Flow Cytometry - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953)ab124953 at 1/10 dilution staining Methionine Aminopeptidase 2 in permeabilized 293T cells by Flow cytometry (red) or a Rabbit IgG (negative control) (green). -
![Immunohistochemistry (Frozen sections) - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953)](http://www.abcam.cn/ps/datasheet/images/124/ab124953/Methionine-Aminopeptidase-2-Primary-antibodies-ab124953-9.jpg)
Immunohistochemistry (Frozen sections) - Anti-Methionine Aminopeptidase 2 antibody [EPR6886(B)] (ab124953)This image is courtesy of an anonymous Abreviewab124953 staining Methionine Aminopeptidase 2 in Mouse spleen tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with Acetone and blocked with 5% Serum for 60 minutes at 25°C. Samples were incubated with primary antibody (1/25 in PBS + 5% goat serum) for 1 hours at 25°C. An Alexa Fluor®488-conjugated Goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
文献
This product has been referenced in:
- Ng JY et al. Tyrosine nitration moderates the peptidase activity of human methionyl aminopeptidase 2. Biochem Biophys Res Commun 440:37-42 (2013). Read more (PubMed: 24041691) »
客户评价及客户问答
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