Metformin hydrochloride (ab120847)

概述

  • 产品名称Metformin hydrochloride
  • 描述
    AMPK activator
  • 生物学描述AMPK activator. Antidiabetic and anticancer activity in vivo. Rapidly crosses the blood-brain barrier.

    Also available in simple stock solutions (ab146725) - add 1 ml of water to get an exact, ready-to-use concentration.
  • 纯度> 99%

性能

    应用

    Our Abpromise guarantee covers the use of ab120847 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Functional Studies Use at an assay dependent concentration.

    Metformin hydrochloride 图像

    • ab1 staining HIF1α in MCF7 cells treated with metformin hydrochloride (ab120847), by ICC/IF. Decrease in HIF1α expression correlates with increased concentration of metformin hydrochloride, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120847 (metformin hydrochloride) in water, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab1 (10 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    Metformin hydrochloride (ab120847)参考文献

    This product has been referenced in:
    • Gallagher EJ & LeRoith D Diabetes, cancer, and metformin: connections of metabolism and cell proliferation. Ann N Y Acad Sci 1243:54-68 (2011). Read more (PubMed: 22211893) »
    • Labuzek K  et al. Quantification of metformin by the HPLC method in brain regions, cerebrospinal fluid and plasma of rats treated with lipopolysaccharide. Pharmacol Rep 62:956-65 (2010). Read more (PubMed: 21098880) »

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"