MetaPath™ Mito Disease 4-Plex Dipstick Array (ab109879)

概述

  • 产品名称MetaPath™ Mito Disease 4-Plex Dipstick Array
  • 样品类型
    Cell culture extracts, Tissue
  • 检测类型Sandwich (quantitative)
  • 检测时间
    0h 30m
  • 种属反应性
    与反应: Human
    不与反应: Mouse
  • 产品概述

    ab109879 (MSX31) is a novel array that allows for the simultaneous quantification of 4 key enzymes whose expression is down-regulated in different mitochondrial diseases: Complex I, Complex IV, PDH and Frataxin. The array is comprised of 4 monoclonal capture antibodies striped onto a dipstick, and the immunocaptured sample is detected using 4 monoclonal detector antibodies that recognize different epitopes on the target enzymes. This array is very rapid (total assay time including sample prep is less than 1 hour) and very sensitive, and as with all Abcam's assays, isolation of mitochondria is not required.

    This array is suitable for testing a variety of sample types. Not only is it useful for measuring samples that are deficient in the 4 enzymes due to genetic mutations, it is also useful for measuring changes in the expression of the enzymes due to drug treatments or other manipulations.

  • 说明

    All components are stable in their provided containers at room temperature out of direct sunlight.

    After diluting the 10X Blocking Buffer to 2X, store at 4°C.

    For long-term storage, all buffers can be stored at 4°C.

  • 经测试应用适用于: Sandwich ELISAmore details
  • 平台Reagents

性能

    应用

    Our Abpromise guarantee covers the use of ab109879 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    Sandwich ELISA Use at an assay dependent dilution.

    MetaPath™ Mito Disease 4-Plex Dipstick Array 图像

    • A typical standard curve for ab109879 using HepG2 cell extract. We suggest using 7 to 8 dipsticks for covering the working range. In this example, the maximum sample load should be 5 µg linear regression above which non-linear regression should be used.
    • Examples of dipsticks from (a) normal fibroblast cells (b) rho0 fibroblast cells which lack mtDNA and hence complexes I and IV only (c) a fibroblast sample from a patient exhibiting a specific deficiency in complex I (d) pure frataxin and (e) buffer control with containing no sample. Data from these sticks was collected using a simple flatbed scanner and ImageJ software.
    • Dipstick assays use the well-established lateral flow concept, whereby capture antibodies are striped onto nitrocellulose membrane and a Whatman paper wicking pad draws the sample through the antibody bands. Detector antibodies, conjugated to gold, are dried in the wells of a 96-well plate. Sample is added to the well, the dipstick inserted, and within minutes the line for each target is revealed as the protein-detector antibody-gold complex binds with the capture antibodies. Multiplexing dipstick assays have multiple target protein lines. A positive control goat anti-mouse antibody line is included on all assays to ensure that adequate wicking of the sample occurred.

    实验方案

    MetaPath™ Mito Disease 4-Plex Dipstick Array (ab109879)参考文献

    This product has been referenced in:
    • Cherry AB  et al. Induced Pluripotent Stem Cells with a Pathological Mitochondrial DNA Deletion. Stem Cells N/A:N/A (2013). Cellular activity ; Human . Read more (PubMed: 23400930) »
    • Ichikawa Y  et al. Disruption of ATP-binding cassette B8 in mice leads to cardiomyopathy through a decrease in mitochondrial iron export. Proc Natl Acad Sci U S A 109:4152-7 (2012). Read more (PubMed: 22375032) »

    See all 2 Publications for this product

    Product Wall

    Thank you for contacting us and reporting the problems you have encountered with the MetaPath™ Mito Disease 4-Plex Dipstick Array (ab109879).

    I am sorry to hear you have been experiencing some problems with the array, however, the Cytoc...

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    No need to fill a form, could you please just provide one of the following : the Abcam order reference number, the PO number, the date of purchase or the address of your lab?
    Thank you.

    Thank you for your message and sorry for the delay of my reply.
    Please find below the answer I received from our production manager :
    > "This is the QC kit lot performed immediately prior to assembly of the retail kit lot GR73147-2. The compo...

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    Thank you for contacting us.

    The lab has sent me the following information:

    The PDH antibody pair in this kit are the only ones that react against mouse. Complex I, IV and frataxin antibodies are for human only. Therefore, the kit's...

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    Thank you for your reply.
    My colleague from the lab has looked at the lot number of the gold and the dipsticks and the QC records.
    Under standard conditions (using cultured cells) the kit should perform as indicated. After looking at the dips...

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    Thank you for sending the screen shots. 
    The lab informed me that the 4plex dipstick does not have a GAM line, there are only 4 lines in the dipstick. 
    Interestingly, they only see 3 peaks in the shots:
    1. 180 - 200
    2. 490 - 505 Read More


    Could you please send us a print-screen of the profile observed after reading dipsticks from a calibration curve (all they need is three profiles), we will be able to determine exactly which peaks you should be looking at.

    Thank you for contacting us.

    On occasion the reader will see more profiles which cannot be seeing with the naked eye on the dipstick. This is due sometimes to imperfections of the nitrocellulose or to uneven wicking. For activity assays the ...

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    Thank you for contacting me with these questions.

    I have had the opportunity to speak with the lab extensively about your issues. Here is what I have learned.

    In regards to the background issue, we know that the gold conjugated antibo...

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    Thank you for contacting us.

    In cases where the control is giving background while the samples have not, it is recommended that you re-try the no sample control. When using the negative control, please be sure to treat the no sample strip exa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"