Anti-Metallophilic Macrophages抗体[MOMA-1] (Biotin) (ab51814)

概述

  • 产品名称
    Anti-Metallophilic Macrophages抗体[MOMA-1] (Biotin)
    参阅全部 Metallophilic Macrophages 一抗
  • 描述
    大鼠单克隆抗体[MOMA-1] to Metallophilic Macrophages (Biotin)
  • 偶联物
    Biotin
  • 特异性
    Does not react with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells.
  • 经测试应用
    适用于: IHC-Frmore details
    不适用于: IHC-P
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Mouse lymph node tissue

  • 阳性对照
    • Mouse spleen
  • 常规说明
    The antigen is differentially induced in in vitro derived macrophages depending on the colony-stimulating factor applied (IL3 > M-CSF > GM-CSF). Distinct macrophage subpopulations of lymphoid organs express the antigen that reacts with this antibody. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer’s patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained. No reactive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine.


    Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.1% Kathon
    Constituents: PBS, 5mg/ml BSA, pH 7.2
  • Concentration information loading...
  • 纯度
    IgG fraction
  • Primary antibody说明
    Monoclonal antibody MOMA-1 is a useful marker for the identification of macrophage subpopulations in various organs, mostly characterized by a high level of non-specific esterase expression. Staining is particularly noteworthy with the metallophilic macrophages adjacent to the marginal zone of the spleen. MOMA-1 is also very suitable for differentiation of non-metallophilic marginal zone macrophages as detected by the monoclonal antibody ER-TR9 (ab51819). In addition, MOMA-1 detects macrophages at inflammatory sites and is positive with Kupffer cells.
  • 克隆
    单克隆
  • 克隆编号
    MOMA-1
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab51814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 0.5 - 1 µg/ml.
  • 应用说明
    Is unsuitable for IHC-P.
  • 靶标

    • 相关性
      Metallophilic macrophages are a subpopulation of mature resident tissue macrophages. They show high non specific esterase activity and can be distinguished from splenic marginal zone macrophages by antibody staining and the lack of FITC-Ficoll uptake.

    Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) 图像

    • ab51814 at 0.5 µg/ml staining mouse spleen; frozen tissue.

    Anti-Metallophilic Macrophages antibody [MOMA-1] (Biotin) (ab51814)参考文献

    This product has been referenced in:
    • Frenkel D  et al. Trypanosoma brucei Co-opts NK Cells to Kill Splenic B2 B Cells. PLoS Pathog 12:e1005733 (2016). Read more (PubMed: 27403737) »
    • Schaefer-Klein JL  et al. Topoisomerase 2 Alpha Cooperates with Androgen Receptor to Contribute to Prostate Cancer Progression. PLoS One 10:e0142327 (2015). WB . Read more (PubMed: 26560244) »

    See all 12 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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