Anti-Metabotropic Glutamate Receptor 7抗体(ab53705)

概述

  • 产品名称
    Anti-Metabotropic Glutamate Receptor 7抗体
    参阅全部 Metabotropic Glutamate Receptor 7 一抗
  • 描述
    兔多克隆抗体to Metabotropic Glutamate Receptor 7
  • 特异性
    ab53705 detects endogenous levels of total Metabotropic Glutamate Receptor 7 protein
  • 经测试应用
    适用于: IP, WB, IHC-P, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide derived from human Metabotropic Glutamate Receptor 7.

  • 阳性对照
    • Human brain tissue and HuvEc cell extracts.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab53705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

靶标

图片

  • Metabotropic Glutamate Receptor 7 was immunoprecipitated using 0.5mg Rat Brain tissue extract, 5µg of Rabbit polyclonal to Metabotropic Glutamate Receptor 7 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Rat Brain tissue extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53705.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 110kDa; Metabotropic Glutamate Receptor 7

  • ab53705 at 1/50 dilution staining Metabotropic Glutamate Receptor 7 in human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.ab53705 at 1/50 dilution staining Metabotropic Glutamate Receptor 7 in human brain by Immunohistochemsitry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
  • All lanes : Anti-Metabotropic Glutamate Receptor 7 antibody (ab53705) at 1/500 dilution

    Lane 1 : HuvEc cell extract
    Lane 2 : HuvEc cell extract with immunising peptide


    Predicted band size : 102 kDa
    Observed band size : 102 kDa

文献

This product has been referenced in:

See 1 Publication for this product

客户评价及客户问答

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Commercial Peroxidase blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Commercial High pH Buffer
Sample
Human Tissue sections (Tendon)
Specification
Tendon
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Sarah Franklin

Verified customer

提交于 Aug 14 2014

Thank you for contacting us.
The immunogen sequence is from C-terminal end.
I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册