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N-terminal synthetic peptide derived from human Metabotropic Glutamate Receptor 5.
Our Abpromise guarantee covers the use of ab53090 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22962568|
|ICC/IF||1/100. PubMed: 19364772|
|IHC-P||1/50 - 1/100.|
|WB||1/300 - 1/1000. Predicted molecular weight: 132 kDa.|
ab53090 staining Metabotropic Glutamate Receptor 5 (red) in rat hippocampla neuron by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 24 hours at 22°C. An Alexa Fluor® 594-conjugated chicken anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Cyan - pre-synaptic marker Bassoon.