Anti-Metabotropic Glutamate Receptor 5抗体(ab27190)

概述

  • 产品名称Anti-Metabotropic Glutamate Receptor 5抗体
    参阅全部 Metabotropic Glutamate Receptor 5 一抗
  • 描述
    兔多克隆抗体to Metabotropic Glutamate Receptor 5
  • 特异性This antibody recognises mGluR5; the staining pattern was cytoplasmic.
  • 经测试应用适用于: IHC-FoFr, ICC/IF, WB, IP, IHC-Pmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Rabbit
  • 免疫原

    Synthetic peptide corresponding to Human Metabotropic Glutamate Receptor 5 aa 1184-1203 (C terminal). This antibody reacts with an epitope located in the C terminal region of mGluR5 which is exactly same in mGluR5a, mGluR5b and mGluR5 precursor.
    Sequence:

    SSPKYDTLIIRDYTQSSSSL

  • 阳性对照
    • Human brain for Immunohistochemistry.

性能

应用

Our Abpromise guarantee covers the use of ab27190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-FoFr 1/100. PubMed: 16979830Please note antigen retrieval is required.
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 135 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
  • 序列相似性Belongs to the G-protein coupled receptor 3 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Glutamate receptor metabotropic 5 antibody
    • GPRC1E antibody
    • Grm5 antibody
    • GRM5_HUMAN antibody
    • Metabotropic glutamate receptor 5 antibody
    • Metabotropic glutamate receptor 5 variant F antibody
    • Metabotropic glutamate receptor 5 variant G antibody
    • Metabotropic glutamate receptor 5 variant H antibody
    • mGlu5 antibody
    • mGluR5 antibody
    • PPP1R86 antibody
    • Protein phosphatase 1 regulatory subunit 86 antibody
    see all

Anti-Metabotropic Glutamate Receptor 5 antibody 图像

  • Ab27190, at a dilution of 1/50, staining mGluR5 in Paraffin embedded formalin fixed human brain tissue section.
  • ICC/IF image of ab27190 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab27190, 1æg/ml) overnight at +4øC. The secondary antibody (green)ÿwas Alexa Fluor© 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor© 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43æM.

Anti-Metabotropic Glutamate Receptor 5 antibody (ab27190)参考文献

This product has been referenced in:
  • Matosin N  et al. Metabotropic glutamate receptor 5 binding and protein expression in schizophrenia and following antipsychotic drug treatment. Schizophr Res 146:170-6 (2013). Read more (PubMed: 23462049) »
  • Gleason KA  et al. Susceptibility of the adolescent brain to cannabinoids: long-term hippocampal effects and relevance to schizophrenia. Transl Psychiatry 2:e199 (2012). Read more (PubMed: 23188199) »

See all 4 Publications for this product

Product Wall

Thank you for our enquiry. I am sorry to confirm that as far as we are aware, this antibody has not specifically been tested on brain samples in western blot so we are not able to provide this information from testing procedures. I can suggest ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Recombinant protein (HEK cell transfected with rat mGluR5)
Loading amount 20 µg
Specification HEK cell transfected with rat mGluR5
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. sriharsha kantamneni

Verified customer

提交于 May 02 2007

Thank you for your enquiry. I am sorry for the delay to your enquiry. I have been in touch with the source of this antibody and I can tell you that PDE10A (ab14622) was tested using rat, mouse and human PDE10A variants (PDE10A1 and DE10A2). However...

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Thank you for your enquiry. I apologise for the delay in responding to your message. We have a high number of enquiries at the moment, and it has taken some time to obtain the information for you. The PDE10A antibody(ab14622) does not cross react w...

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I was informed a few minutes ago by the laboratory that the antigen is a peptide from the C-Terminus, which is exactly same in mGluR5a, mGluR5b and mGluR5 precursor. I apologise for the delay in finding out this information and hope it will be useful....

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Thank you for your enquiry. Following correspondence with the source of this antibody I can tell you that they commented that this antibody gave relatively low background staining. They also mentioned that the intensity of staining observed by this ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"