Anti-Metabotropic Glutamate Receptor 2抗体[mG2Na-s] (ab15672)

概述

  • 产品名称
    Anti-Metabotropic Glutamate Receptor 2抗体[mG2Na-s]
    参阅全部 Metabotropic Glutamate Receptor 2 一抗
  • 描述
    小鼠单克隆抗体[mG2Na-s] to Metabotropic Glutamate Receptor 2
  • 特异性
    This antibody recognizes the metabotrophic glutamate receptor 2, but not metabotrophic glutamate receptor 3.
  • 经测试应用
    适用于: IHC-Fr, WB, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Fusion protein corresponding to Metabotropic Glutamate Receptor 2. Fusion protein contains a 47 amino acid sequence of the N terminal of the Metabotrophic Glutamate Receptor 2

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: None
    Constituents: PBS, pH 7.4 sterile filtered
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    单克隆
  • 克隆编号
    mG2Na-s
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 1 µg/ml. See reference Neki et al, Neurosci Letts.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 104 kDa). See reference Neki et al, Neurosci Letts.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent dilution.

靶标

  • 功能
    Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
  • 组织特异性
    Widely expressed in different regions of the adult brain as well as in fetal brain.
  • 序列相似性
    Belongs to the G-protein coupled receptor 3 family.
  • 细胞定位
    Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMPA selective glutamate receptor 2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate metabotropic receptor 2 antibody
    • Glutamate receptor homolog antibody
    • Glutamate receptor metabotropic 2 antibody
    • GPRC1B antibody
    • GRM2 antibody
    • GRM2_HUMAN antibody
    • Metabotropic glutamate receptor 2 antibody
    • mGlu2 antibody
    • mGluR2 antibody
    • OTTHUMP00000210984 antibody
    • OTTHUMP00000210986 antibody
    see all

图片

  • Anti-Metabotropic Glutamate Receptor 2 antibody [mG2Na-s] (ab15672) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • Immunohistochemical analysis of mouse cortical neurons, staining Metabotropic Glutamate Receptor 2 with ab15672. Donkey anti-mouse 6-nm colloidal gold particles were used to detect staining. Images were visualized by electron microscopy.

文献

This product has been referenced in:
  • Cao DY  et al. EXPRESS: Histone hyperacetylation modulates spinal type II metabotropic glutamate receptor alleviating stress-induced visceral hypersensitivity in female rats. Mol Pain 12:N/A (2016). WB . Read more (PubMed: 27385724) »
  • Moreno JL  et al. Allosteric signaling through an mGlu2 and 5-HT2A heteromeric receptor complex and its potential contribution to schizophrenia. Sci Signal 9:ra5 (2016). Read more (PubMed: 26758213) »

See all 13 Publications for this product

客户评价及客户问答

Application
Western blot
Sample
Human Tissue lysate - whole (Human brain lysate)
Gel Running Conditions
Reduced Denaturing (Samples heated 5 min at 37 degrees, 4%-15% gel)
Loading amount
10 µg
Specification
Human brain lysate
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 11 2016

You are correct, this antibody recognizes a portion of the N-terminus of mGluR2.

I am glad to read that most of the antibodies you ordered are giving good results. For the two that are not, could you please send some details of the samples and protocols and results? In particular, we would like to know the species, the time in PFA,...

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Gracias por tu respuesta.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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