Anti-Metabotropic Glutamate Receptor 2抗体[mG2Na-s] (ab15672)

概述

  • 产品名称Anti-Metabotropic Glutamate Receptor 2抗体[mG2Na-s]
    参阅全部 Metabotropic Glutamate Receptor 2 一抗
  • 描述
    小鼠单克隆抗体[mG2Na-s] to Metabotropic Glutamate Receptor 2
  • 特异性This antibody recognizes the metabotrophic glutamate receptor 2, but not metabotrophic glutamate receptor 3.
  • 经测试应用适用于: IHC-Fr, WB, ICC/IF, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat
  • 免疫原

    Fusion protein corresponding to Metabotropic Glutamate Receptor 2. Fusion protein contains a 47 amino acid sequence of the N terminal of the Metabotrophic Glutamate Receptor 2

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS, pH 7.4 sterile filtered
  • Concentration information loading...
  • 纯度Protein A purified
  • 克隆单克隆
  • 克隆编号mG2Na-s
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15672 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 1 µg/ml. See reference Neki et al, Neurosci Letts.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 104 kDa). See reference Neki et al, Neurosci Letts.
ICC/IF Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent dilution.

靶标

  • 功能Receptor for glutamate. The activity of this receptor is mediated by a G-protein that inhibits adenylate cyclase activity. May mediate suppression of neurotransmission or may be involved in synaptogenesis or synaptic stabilization.
  • 组织特异性Widely expressed in different regions of the adult brain as well as in fetal brain.
  • 序列相似性Belongs to the G-protein coupled receptor 3 family.
  • 细胞定位Cell membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • AMPA selective glutamate receptor 2 antibody
    • GLUR2 antibody
    • GLURB antibody
    • Glutamate metabotropic receptor 2 antibody
    • Glutamate receptor homolog antibody
    • Glutamate receptor metabotropic 2 antibody
    • GPRC1B antibody
    • GRM2 antibody
    • GRM2_HUMAN antibody
    • Metabotropic glutamate receptor 2 antibody
    • mGlu2 antibody
    • mGluR2 antibody
    • OTTHUMP00000210984 antibody
    • OTTHUMP00000210986 antibody
    see all

Anti-Metabotropic Glutamate Receptor 2 antibody [mG2Na-s] 图像

  • Anti-Metabotropic Glutamate Receptor 2 antibody [mG2Na-s] (ab15672) at 1 µg/ml + Brain (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 104 kDa
    Observed band size : 99 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • Immunohistochemical analysis of mouse cortical neurons, staining Metabotropic Glutamate Receptor 2 with ab15672. Donkey anti-mouse 6-nm colloidal gold particles were used to detect staining. Images were visualized by electron microscopy.

Anti-Metabotropic Glutamate Receptor 2 antibody [mG2Na-s] (ab15672)参考文献

This product has been referenced in:
  • Kurita M  et al. Repressive epigenetic changes at the mGlu2 promoter in frontal cortex of 5-HT2A knockout mice. Mol Pharmacol 83:1166-75 (2013). Read more (PubMed: 23508685) »
  • Konermann S  et al. Optical control of mammalian endogenous transcription and epigenetic states. Nature 500:472-6 (2013). Read more (PubMed: 23877069) »

See all 12 Publications for this product

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Human brain lysate)
Gel Running Conditions Reduced Denaturing (Samples heated 5 min at 37 degrees, 4%-15% gel)
Loading amount 10 µg
Specification Human brain lysate
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Oct 11 2016

You are correct, this antibody recognizes a portion of the N-terminus of mGluR2.

I am glad to read that most of the antibodies you ordered are giving good results. For the two that are not, could you please send some details of the samples and protocols and results? In particular, we would like to know the species, the time in PFA,...

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Gracias por tu respuesta.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"