Anti-Metabotropic Glutamate Receptor 1抗体(ab82211)

概述

  • 产品名称
    Anti-Metabotropic Glutamate Receptor 1抗体
    参阅全部 Metabotropic Glutamate Receptor 1 一抗
  • 描述
    兔多克隆抗体to Metabotropic Glutamate Receptor 1
  • 经测试应用
    适用于: WB, IHC-P, ICC/IF, IHC-Fr, IHC-FoFrmore details
  • 种属反应性
    与反应: Mouse, Rat, Chicken, Human
  • 免疫原

    Synthetic peptide:

    SSVPSSPVSESVL

    , corresponding to amino acids 1159-1171 of Human Metabotropic Glutamate Receptor 1

  • 阳性对照
    • Human brain lysate, rat dorsal root ganglion, and rat brain (cingulate cortex).

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82211 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 133 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/100 - 1/500.
IHC-Fr 1/200.
IHC-FoFr Use at an assay dependent concentration.

靶标

图片

  • Anti-Metabotropic Glutamate Receptor 1 antibody (ab82211) at 1/1000 dilution + human brain lysate at 20 µg

    Secondary
    AP-conjugated secondary at 1/5000 dilution

    Predicted band size : 133 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence labeling of Metabotropic Glutamate Receptor 1 in rat brain (cingulate cortex). Using ab82211 (red color). DAPI was used to counter-stain cell nuclei (blue color). Working dilution is 1/100-1/500.
  • Rat dorsal root ganglion. Tissue was fixed in 4% paraformaldehyde and cut into 10 micron sections. ab82211 was used at a dilution of 1/200 and secondary antibody at a dilution of 1/5000.
  • ab82211 staining Metabotropic Glutamate Receptor 1 in rat cerebellum tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using Triton X-100, blocked with 7% serum for 3 hours at 25°C, then incubated with ab82211 at a 1/200 dilution for 10 hours at 25°C. The secondary used was an undiluted HRP conjugated goat polyclonal.

    See Abreview

文献

This product has been referenced in:
  • Valiya Veettil M  et al. Glutamate secretion and metabotropic glutamate receptor 1 expression during Kaposi's sarcoma-associated herpesvirus infection promotes cell proliferation. PLoS Pathog 10:e1004389 (2014). Human . Read more (PubMed: 25299066) »
  • Sylantyev S  et al. Spike-driven glutamate electrodiffusion triggers synaptic potentiation via a homer-dependent mGluR-NMDAR link. Neuron 77:528-41 (2013). IHC-P . Read more (PubMed: 23395378) »

See all 2 Publications for this product

客户评价及客户问答

Application
Immunohistochemistry (Frozen sections)
Blocking step
(agent) for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample
Cat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 07 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 07 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Dr. Yuhua Liu

Verified customer

提交于 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Sample
Rat Cell (brain)
Specification
brain
Permeabilization
Yes - 0.3% triton
Fixative
Methanol
Username

Abcam user community

Verified customer

提交于 Sep 12 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Chicken Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.1% trition
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 11 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Mouse Tissue sections (brainstem)
Specification
brainstem
Permeabilization
Yes - 0.3% triton
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Jul 16 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate sodium
Sample
Rat Tissue sections (brainstem)
Specification
brainstem
Permeabilization
No
Fixative
Paraformaldehyde
Username

Wei Shi

Verified customer

提交于 Jul 16 2013

I performed a search of some other anti - metabotropic glutamate receptor 1 antibodies, and it seems the glutamate receptor can run between ˜110 - 130 kDa depending on the post-translational modification in the tested samples. Thus, the 110 kDa band th...

Read More

Thank you very much for your email.

Please do not hesitate to contact me if this antibody does not work.

I hope this information will be helpful. Should you have nay question please do not hesitate to contact me.

1-10 of 17 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册