Anti-Met Enkephalin抗体(ab22620)


  • 产品名称
    Anti-Met Enkephalin抗体
  • 描述
    兔多克隆抗体to Met Enkephalin
  • 宿主
  • 经测试应用
    适用于: ICC/IF, IHC-P, IHC-Rmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to Met Enkephalin conjugated to Keyhole Limpet Haemocyanin (KLH) (Glutaraldehyde). The conjugate was dialysed to remove excess conjugating agent prior to immunisation.

  • 阳性对照
    • Typical test tissues included rat and human brain, rat spinal cord, pig duodenum, guinea pig ileum (enteric nervous system) and human phaeochromocytoma (adrenomedullary tumour)



Our Abpromise guarantee covers the use of ab22620 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-R 1/500 - 1/4000. (Using overnight incubation and ABC/PAP procedure). Dilutions up to 1/10,000 have been achieved under optimised conditions. 1/200-1/4000, using overnight incubation and indirect immunofluorescence procedure. All standard crosslinking agents may be used, but formaldehyde based solutions are recommended. Frozen sections and Vibratome slices are recommended for tracttracing.


  • 功能
    Met- and Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. PENK(114-133) and PENK(237-258) increase glutamate release in the striatum. PENK(114-133) decreases GABA concentration in the striatum.
  • 序列相似性
    Belongs to the opioid neuropeptide precursor family.
  • 翻译后修饰
    The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
  • 细胞定位
  • Information by UniProt
  • 数据库链接
  • 别名
    • Leu-enkephalin antibody
    • Met-enkephalin antibody
    • Met-enkephalin-Arg-Gly-Leu antibody
    • Met-enkephalin-Arg-Phe antibody
    • Methionine enkephalin antibody
    • OGF antibody
    • Opioid growth factor antibody
    • PENK antibody
    • PENK(114-133) antibody
    • PENK(143-183) antibody
    • PENK(237-258) antibody
    • PENK_HUMAN antibody
    • PPA antibody
    • Proenkephalin A antibody
    • Proenkephalin antibody
    • Proenkephalin-A antibody
    • Synenkephalin antibody
    see all


  • ICC/IF image of ab22620 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22620, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab22620 (4µg/ml) staining met enkephalin in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of seminal vesicles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.


This product has been referenced in:
  • Hollis F  et al. Mitochondrial function in the brain links anxiety with social subordination. Proc Natl Acad Sci U S A 112:15486-91 (2015). IHC-Fr ; Rat . Read more (PubMed: 26621716) »

See 1 Publication for this product


Immunohistochemistry (Frozen sections)
Rat Tissue sections (Brain slices (Striatum))
Yes - 15 minutes in PBS-Triton 0.2%
Brain slices (Striatum)
Blocking step
PBS-Tween 0.05% + Goat Serum 10% + 0.3M Glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C

Abcam user community

Verified customer

提交于 Jan 23 2018

Thank you for your inquiry.

I can confirm that ab22620 was tested and is guaranteed for human samples.

If your customer would like to use this antibody with another species, I will be happy to issue a testing discount.

I h...

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Thanks for your call today. I ran a BLAST with the immunogen of ab98128 against the Met and Leu sequences on SwissProt, and though the immunogen contains the entire Met sequence (YGGFM), it does not contain the full Leu sequence (only YGGF). So, this a...

Read More