Anti-Met (c-Met)抗体(ab137654)
Key features and details
- Rabbit polyclonal to Met (c-Met)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Met (c-Met)抗体
参阅全部 Met (c-Met) 一抗 -
描述
兔多克隆抗体to Met (c-Met) -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide, corresponding to a region witin C terminal amino acids 1329-1390 of Human Met (c-Met).
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137654于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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WB |
1/500 - 1/3000. Predicted molecular weight: 158 kDa.
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说明 |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Suggested antigen retrieval using heat mediated 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
WB
1/500 - 1/3000. Predicted molecular weight: 158 kDa. |
靶标
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功能
Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival. -
疾病相关
Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
Note=Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. -
结构域
The kinase domain is involved in SPSB1 binding. -
翻译后修饰
Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 4233 Human
- Omim: 164860 Human
- SwissProt: P08581 Human
- Unigene: 132966 Human
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别名
- AUTS9 antibody
- c met antibody
- D249 antibody
see all
图片
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All lanes : Anti-Met (c-Met) antibody (ab137654) at 1/1000 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : Met (c-Met) Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 158 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted? -
Anti-Met (c-Met) antibody (ab137654) at 1/1000 dilution + Hela S3 whole cell lysate at 30 µg
Predicted band size: 158 kDa
5% SDS PAGE -
Immunohistochemical analysis of paraffin embedded Cal27 xenograft, labelling Met (c-Met) with ab137654 at 1/100 dilution.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab137654 被引用在 4 文献中.
- Liu C et al. Upregulation of LINC00511 expression by DNA hypomethylation promotes the progression of breast cancer. Gland Surg 10:1418-1430 (2021). PubMed: 33968693
- Chen X et al. Curcumin Inhibits HGF-Induced EMT by Regulating c-MET-Dependent PI3K/Akt/mTOR Signaling Pathways in Meningioma. Evid Based Complement Alternat Med 2021:5574555 (2021). PubMed: 34408780
- Endo Y et al. T-DM1-resistant cells gain high invasive activity via EGFR and integrin cooperated pathways. MAbs 10:1003-1017 (2018). PubMed: 30130447
- Gerdes MJ et al. Highly multiplexed single-cell analysis of formalin-fixed, paraffin-embedded cancer tissue. Proc Natl Acad Sci U S A 110:11982-7 (2013). ICC/IF ; Human . PubMed: 23818604