- 数据表
- 具体的参考文献 (2)
- 实验方案
概述
- 产品名称Anti-Met (c-Met)抗体
参阅全部 Met (c-Met) 一抗 - 描述山羊多克隆抗体to Met (c-Met)
- 经测试应用适用于: ICC/IF, Neutralising, ELISA, WBmore details
- 种属反应性与反应: Mouse, Human
- 免疫原
Recombinant fragment (extracellular domain)(Human).
- 常规说明
The detection limit in immunoblotting for recombinant human HGF R is approximately 5 ng/lane under non-reducing and reducing conditions. Both the alpha and beta chains of HGF R are detected by this antibody under reducing conditions. The detection limit in ELISA for recombinant human HGF R is approximately 0.16 ng/well.
性能
- 形式Liquid
- 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
- 存储溶液Constituents: PBS, 0.5% Trehalose
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Concentration information loading... - 纯度Immunogen affinity purified
- Primary antibody说明The detection limit in immunoblotting for recombinant human HGF R is approximately 5 ng/lane under non-reducing and reducing conditions. Both the alpha and beta chains of HGF R are detected by this antibody under reducing conditions. The detection limit in ELISA for recombinant human HGF R is approximately 0.16 ng/well.
- 克隆多克隆
- 同种型IgG
- 研究领域
相关产品
- Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab10728 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| Neutralising | Use a concentration of 0.5 - 2 µg/ml. ab10728 has the ability to neutralize receptor-ligand interaction. Approximately 0.5-2 mg/mL of the antibody will block 50% of the binding of recombinant human HGF (5 ng/mL) to immobilized recombinant human HGF R/Fc Chimera (100 mL of a 1 mg/mL solution coated in each well) in an ELISA. 10 mg/mL of the antibody will block 90% of binding. | |
| ELISA | Use a concentration of 0.5 - 1 µg/ml. | |
| WB | Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 129 kDa. |
靶标
- 功能Receptor for hepatocyte growth factor and scatter factor. Has a tyrosine-protein kinase activity. Functions in cell proliferation, scattering, morphogenesis and survival.
- 疾病相关Note=Activation of MET after rearrangement with the TPR gene produces an oncogenic protein.
Note=Defects in MET may be associated with gastric cancer.
Defects in MET are a cause of hepatocellular carcinoma (HCC) [MIM:114550].
Defects in MET are a cause of renal cell carcinoma papillary (RCCP) [MIM:605074]. It is a subtype of renal cell carcinoma tending to show a tubulo-papillary architecture formed by numerous, irregular, finger-like projections of connective tissue. Renal cell carcinoma is a heterogeneous group of sporadic or hereditary carcinoma derived from cells of the proximal renal tubular epithelium. It is subclassified into common renal cell carcinoma (clear cell, non-papillary carcinoma), papillary renal cell carcinoma, chromophobe renal cell carcinoma, collecting duct carcinoma with medullary carcinoma of the kidney, and unclassified renal cell carcinoma.
Note=A common allele in the promoter region of the MET shows genetic association with susceptibility to autism in some families. Functional assays indicate a decrease in MET promoter activity and altered binding of specific transcription factor complexes.
Note=MET activating mutations may be involved in the development of a highly malignant, metastatic syndrome known as cancer of unknown primary origin (CUP) or primary occult malignancy. Systemic neoplastic spread is generally a late event in cancer progression. However, in some instances, distant dissemination arises at a very early stage, so that metastases reach clinical relevance before primary lesions. Sometimes, the primary lesions cannot be identified in spite of the progresses in the diagnosis of malignancies. - 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 3 IPT/TIG domains.
Contains 1 protein kinase domain.
Contains 1 Sema domain. - 结构域The kinase domain is involved in SPSB1 binding.
- 翻译后修饰Dephosphorylated by PTPRJ at Tyr-1349 and Tyr-1365.
- 细胞定位Membrane.
- Information by UniProt
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数据库链接
- Entrez Gene: 4233 Human
- Entrez Gene: 17295 Mouse
- Omim: 164860 Human
- SwissProt: P08581 Human
- SwissProt: P16056 Mouse
- Unigene: 132966 Human
- Unigene: 86844 Mouse
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别名
- AUTS9 antibody
- c met antibody
- D249 antibody
see all
Anti-Met (c-Met) antibody 图像
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Immunocytochemistry/ Immunofluorescence - Anti-Met (c-Met) antibody (ab10728)This image is courtesy of an anonymous Abreviewab10728 staining mouse muscle cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton prior to blocking with 1% serum for 30 minutes at 25°C. The primary antibody was diluted 1/50 and incubated with the sample for 1 hour at 25°C. An Alexa Fluor® 488 conjugated chicken anti-goat antibody was used as the secondary.
Anti-Met (c-Met) antibody (ab10728)参考文献
This product has been referenced in:
- Yan K et al. MicroRNA-34a Inhibits the Proliferation and Metastasis of Osteosarcoma Cells Both In Vitro and In Vivo. PLoS One 7:e33778 (2012). WB ; Mouse . Read more (PubMed: 22457788) »
- Xu W et al. Cyclosporine A stimulated hair growth from mouse vibrissae follicles in an organ culture model. J Biomed Res 26:372-80 (2012). Mouse . Read more (PubMed: 23554774) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"