Anti-Mesp1抗体(ab77013)

概述

  • 产品名称
  • 描述
    小鼠单克隆抗体to Mesp1
  • 经测试应用
    适用于: ELISA, WB, ICC/IF, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-63 of Human Mesp1

  • 阳性对照
    • FHs 173 WE cell lysate; Recombinant tagged full length human Mesp1.
  • 常规说明

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

性能

应用

Our Abpromise guarantee covers the use of ab77013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

靶标

  • 功能
    Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
  • 序列相似性
    Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位
    Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHc5 antibody
    • Class C basic helix-loop-helix protein 5 antibody
    • Mesoderm posterior 1 antibody
    • Mesoderm posterior 1 homolog (mouse) antibody
    • Mesoderm posterior 1 homolog antibody
    • Mesoderm posterior protein 1 antibody
    • MESP 1 antibody
    • Mesp1 antibody
    • MESP1_HUMAN antibody
    • MGC10676 antibody
    • MGC159208 antibody
    • MGC159210 antibody
    • OTTMUSP00000030151 antibody
    see all

图片

  • Anti-Mesp1 antibody (ab77013) at 1 µg/ml + FHs 173 WE cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

文献

ab77013 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Sample
Mouse Cell (E7.5 embryo)
Specification
E7.5 embryo
Permeabilization
Yes - 0.1% TritonX100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Many thanks for contacting us last week in regards to antibodies raised against Mesp1.

As discussed over the phone, the anti-Mesp1 antibody (ab77013) has as yet not been used in ICC. This does not mean that it would not work, simply that we ha...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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