Anti-Mesp1抗体(ab77013)

概述

  • 产品名称Anti-Mesp1抗体
    参阅全部 Mesp1 一抗
  • 描述
    小鼠单克隆抗体to Mesp1
  • 经测试应用适用于: ELISA, WB, ICC/IF, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1-63 of Human Mesp1

  • 阳性对照
    • FHs 173 WE cell lysate; Recombinant tagged full length human Mesp1.

性能

应用

Our Abpromise guarantee covers the use of ab77013 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent concentration.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 29 kDa.
ICC/IF Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.

靶标

  • 功能Transcription factor. Plays a role in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Defines the rostrocaudal patterning of the somites by participating in distinct Notch pathways.
  • 序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • bHLHc5 antibody
    • Class C basic helix-loop-helix protein 5 antibody
    • Mesoderm posterior 1 antibody
    • Mesoderm posterior 1 homolog (mouse) antibody
    • Mesoderm posterior 1 homolog antibody
    • Mesoderm posterior protein 1 antibody
    • MESP 1 antibody
    • Mesp1 antibody
    • MESP1_HUMAN antibody
    • MGC10676 antibody
    • MGC159208 antibody
    • MGC159210 antibody
    • OTTMUSP00000030151 antibody
    see all

Anti-Mesp1 antibody 图像

  • Anti-Mesp1 antibody (ab77013) at 1 µg/ml + FHs 173 WE cell lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 29 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

Anti-Mesp1 antibody (ab77013)参考文献

ab77013 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Sample Mouse Cell (E7.5 embryo)
Specification E7.5 embryo
Permeabilization Yes - 0.1% TritonX100
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Oct 24 2014

Many thanks for contacting us last week in regards to antibodies raised against Mesp1.

As discussed over the phone, the anti-Mesp1 antibody (ab77013) has as yet not been used in ICC. This does not mean that it would not work, simply that we ha...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"