概述

  • 产品名称Anti-Mesothelin抗体
    参阅全部 Mesothelin 一抗
  • 描述
    山羊多克隆抗体to Mesothelin
  • 特异性This antibody is expected to recognize isoform 1 and 2 (NP_005814.2; NP_037536.2).
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide:

    CPSGKKAREIDES

    , corresponding to internal sequence amino acids 302-314 of Human Mesothelin (NP_005814.2; NP_037536.2).

  • 阳性对照
    • Human placenta lysate.

性能

应用

Our Abpromise guarantee covers the use of ab99474 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 69 kDa). An approximately 70 kDa band was observed in Human Placenta lysates. An additional band of unknown identity was also consistently observed at 150 kDa. This band was successfully blocked by incubation with the immunizing peptide.

靶标

  • 功能Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • 组织特异性Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • 疾病相关Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • 序列相似性Belongs to the mesothelin family.
  • 翻译后修饰Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • 细胞定位Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • Msln antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

Anti-Mesothelin antibody 图像

  • Anti-Mesothelin antibody (ab99474) at 0.3 µg/ml + Human placenta lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 69 kDa

Anti-Mesothelin antibody (ab99474)参考文献

ab99474 has not yet been referenced specifically in any publications.

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