概述

  • 产品名称
    Anti-Mesothelin抗体
    参阅全部 Mesothelin 一抗
  • 描述
    兔多克隆抗体to Mesothelin
  • 特异性
    Detects isoform 2.
  • 经测试应用
    适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Horse, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 539-588 (QKLLGPHVEG LKAEERHRPV RDWILRQRQD DLDTLGLGLQ GGIPNGYLVL) of Human Mesothelin (NP_005814).

  • 阳性对照
    • MCF7 cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab83658 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 32 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

靶标

  • 功能
    Membrane-anchored forms may play a role in cellular adhesion.
    Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
  • 组织特异性
    Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
  • 疾病相关
    Note=Antibodies against MSLN are detected in patients with mesothelioma and ovarian cancer.
  • 序列相似性
    Belongs to the mesothelin family.
  • 翻译后修饰
    Both MPF and the cleaved form of mesothelin are N-glycosylated.
    Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
  • 细胞定位
    Secreted and Cell membrane. Golgi apparatus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CAK 1 antibody
    • CAK1 antibody
    • CAK1 antigen antibody
    • cleaved form antibody
    • Megakaryocyte potentiating factor antibody
    • Mesothelin antibody
    • Mesothelin isoform 1 precursor antibody
    • MPF antibody
    • Msln antibody
    • MSLN_HUMAN antibody
    • Pre pro megakaryocyte potentiating factor antibody
    • Pre-pro-megakaryocyte-potentiating factor antibody
    • SMR antibody
    • SMRP antibody
    • Soluble MPF mesothelin related protein antibody
    see all

图片

  • Anti-Mesothelin antibody (ab83658) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 32 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)

实验方案

文献

ab83658 has not yet been referenced specifically in any publications.

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