概述

  • 产品名称Anti-MERTK抗体
    参阅全部 MERTK 一抗
  • 描述
    兔多克隆抗体to MERTK
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Mouse MERTK.

    (Peptide available as ab106208.)

  • 阳性对照
    • This antibody gave a positive signal in the following whole cell lysates: NIH 3T3; RAW 264.7.

性能

应用

Our Abpromise guarantee covers the use of ab95925 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 110 kDa).
ICC/IF Use a concentration of 10 µg/ml.

靶标

  • 功能In case of filovirus infection, seems to function as a cell entry factor.
  • 组织特异性Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines.
  • 疾病相关Defects in MERTK are the cause of retinitis pigmentosa type 38 (RP38) [MIM:613862]. RP38 is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.
    Contains 2 fibronectin type-III domains.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 protein kinase domain.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • c MER antibody
    • c mer proto oncogene tyrosine kinase antibody
    • c-mer antibody
    • cMER antibody
    • cmer protooncogene tyrosine kinase antibody
    • Eyk antibody
    • MER antibody
    • MER receptor tyrosine kinase antibody
    • MERK antibody
    • MERPEN antibody
    • Mertk antibody
    • MERTK c-mer proto-oncogene tyrosine kinase antibody
    • MERTK_HUMAN antibody
    • MGC133349 antibody
    • nmf12 antibody
    • Nyk antibody
    • Proto oncogene tyrosine protein kinase MER antibody
    • Proto oncogene tyrosine protein kinase MER precursor antibody
    • Proto-oncogene c-Mer antibody
    • Receptor tyrosine kinase MerTK antibody
    • RP38 antibody
    • STK kinase antibody
    • Tyrosine-protein kinase Mer antibody
    see all

Anti-MERTK antibody 图像

  • All lanes : Anti-MERTK antibody (ab95925) at 1 µg/ml

    Lane 1 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 2 : RAW 264.7 (Mouse leukaemic monocyte macrophage cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 110 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
    MERTK protein contains a number of potential glycosylation sites (SwissProt), which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab95925 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95925 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-MERTK antibody (ab95925)参考文献

ab95925 has not yet been referenced specifically in any publications.

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