概述

  • 产品名称
  • 描述
    山羊多克隆抗体to MEPCE
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Rat, Human
    预测可用于: Mouse, Horse, Dog, Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide:

    KQQRKFQYGNYCK

    , corresponding to internal sequence amino acids 408-420 of Human MEPCE (NP_062552.2).

  • 阳性对照
    • Rat testis lysates.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline, pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    ab77329 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab77329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
ELISA Use at an assay dependent dilution.

靶标

  • 相关性
    MEPCE belongs to the methyltransferase superfamily and contains one BIN3 domain. It is an S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA, leading to stabilize it. MEPCE is expressed in chronic myeloid leukemia cells, adrenal gland, brain, cerebellum, kidney, lung, mammary gland and testis. It is weakly or not expressed in other tissues.
  • 数据库链接
  • 别名
    • 7SK snRNA methylphosphate capping enzyme antibody
    • BCDIN3 antibody
    • Bicoid interacting protein 3 homolog antibody
    • Bin3 bicoid interacting 3 antibody
    • Bin3 bicoid interacting 3 homolog (Drosophila) antibody
    • Bin3 bicoid interacting 3 homolog antibody
    • Bin3 homolog antibody
    • FLJ20257 antibody
    • MePCE antibody
    • Methylphosphate capping enzyme antibody
    see all

图片

  • ab77329 (5µg/ml) staining of MEPCE in paraffin embedded Human Testis following steamed antigen retrieval with citrate buffer pH 6 and AP-staining shows staining of the spermatids.

  • Anti-MEPCE antibody (ab77329) at 1 µg/ml + rat testis lysate in RIPA buffer at 35 µg

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
    Primary incubation was 1 hour. Detected by chemiluminescence.

实验方案

文献

ab77329 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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