Anti-Menin抗体- ChIP Grade (ab2605)

概述

  • 产品名称Anti-Menin抗体- ChIP Grade
    参阅全部 Menin 一抗
  • 描述
    兔多克隆抗体to Menin - ChIP Grade
  • 经测试应用适用于: ChIP, IHC-P, ICC/IF, IP, WBmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide (Human) - which represents a portion of the C-terminus of human MEN1.

  • 阳性对照
    • 293T cell lysate.

性能

应用

Our Abpromise guarantee covers the use of ab2605 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ChIP Use a concentration of 1 - 4 µg/ml.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC/IF 1/300 - 1/600.
IP Use at 2-10 µg/mg of lysate.
WB 1/1000 - 1/10000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

靶标

  • 功能Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression (By similarity). May be involved in DNA repair.
  • 组织特异性Ubiquitous.
  • 疾病相关Defects in MEN1 are the cause of familial multiple endocrine neoplasia type I (MEN1) [MIM:131100]. Autosomal dominant disorder characterized by tumors of the parathyroid glands, gastro-intestinal endocrine tissue, the anterior pituitary and other tissues. Cutaneous lesions and nervous-tissue tumors can exist. Prognosis in MEN1 patients is related to hormonal hypersecretion by tumors, such as hypergastrinemia causing severe peptic ulcer disease (Zollinger-Ellison syndrome, ZES), primary hyperparathyroidism, and acute forms of hyperinsulinemia.
    Defects in MEN1 are the cause of familial isolated hyperparathyroidism (FIHP) [MIM:145000]; also known as hyperparathyroidism type 1 (HRPT1). FIHP is an autosomal dominant disorder characterized by hypercalcemia, elevated parathyroid hormone (PTH) levels, and uniglandular or multiglandular parathyroid tumors.
  • 翻译后修饰Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 细胞定位Nucleus. Concentrated in nuclear body-like structures. Relocates to the nuclear matrix upon gamma irradiation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MEA 1 antibody
    • MEA1 antibody
    • MEN 1 antibody
    • Men1 antibody
    • MEN1_HUMAN antibody
    • Menin antibody
    • Multiple Endocrine Adenomatosis 1 antibody
    • Multiple Endocrine Neoplasia 1 antibody
    • SCG 2 antibody
    • SCG2 antibody
    • Suppressor Candidate Gene 2 antibody
    • Wermer syndrome antibody
    • ZES antibody
    • Zollinger Ellison Syndrome antibody
    see all

Anti-Menin antibody - ChIP Grade 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Menin with ab2605 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 68 kDa
  • Detection of menin in 293 cells using ab2605 (2ug/ml). Secondary antibody is Cy3 conjugated anti rabbit IgG.
  • Ab2605 staining Human normal placenta. Staining is localized to the nucleus.
    Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Anti-Menin antibody - ChIP Grade (ab2605)参考文献

This product has been referenced in:

See all 9 Publications for this product

Product Wall


The following references may be useful to you for specific primers for positive and negative loci associated with Menin:

ab31902 and ab32963: Menin highly enriched in PCNA PRO 2, weakly enriched in PCNA PRO 1 according to PMID:18940868 ht...

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I have issued a free of charge replacement with the order number 1042072.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that typically our Abpromise guarantee hold...

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Thanks for your reply.

You are correct that lot GR21097-1 is a new lot we have in stock. We are not able to stock small sample aliqots of our antibodies unfortunately. However, since a vial of ab21873 you previously purchased is not working a...

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Thank you for your enquiry. I am sorry to hear of the trouble you are having with lot 937205 in ChIP. I looked in our records and there was one other complaint of a similar nature regarding ChIP with this lot of ab21873. We do have a new lot in stock n...

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Thank you for contacting us.

I have looked into whether the anti-Menin antibody (ab2605)would be suitable for the experiments you are hoping to perform. Unfortunately, the immunogen used to raise this antibody is directed against the C-termin...

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Thank you for your enquiry. The epitope recognized by ab2605 maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Unfortunately, t...

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Thank you for your enquiry. The epitope recognized by this product maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering given in entry NP_000235.2 (GeneID 4221). Unfortunat...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hella cell)
Specification Hella cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Jiang Hu

Verified customer

提交于 Jun 01 2007

Thank for your patience. I have received a reply from the originator of ab2605. The epitope recognized by ab2605 maps to a region between residue 575 and the C-terminus (residue 615) of human Multiple Endocrine Neoplasia 1 using the numbering g...

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Thank you for your enquiry and my apologies for the delay in replying to you, I have just received further information from the lab to answer your questions. We are not sure of the exact dilution used for the image on the datasheet, it is between 1/...

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