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ab110414 (MS620) contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:
Outer Membrane - Porin ab14734 (MSA03)
Intermembrane Space - Cytochrome c ab110325 (MSA06)
Inner Membrane - Complex Va ab110273 (MS502) and Complex III Core 1 ab110252 (MS303)
Matrix Space - Cyclophilin D ab110324 (MSA04)
This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Va, 49kDa Complex III Core 1, and 21kDa Cyclophilin D.
|Cocktail of 5 Antibodies||1 x 255µg|
|Rat liver mitochondria control||1 x 50µg|
Our Abpromise guarantee covers the use of ab110414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 10.2 µg/ml. The antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of10.2 µg/mL for Western blotting.|
Sample: Mouse whole cell lysate (3T3 SA cell line)
Loading amount: 20µg
Gel Running conditions: reduced denaturating (4-12% gradient gel)
Blocking step: 30 min in 5% milk at room temperature
Incubation time: Overnight at 4°C or 1 hour at room temperature
Secondary antibody: Goat anti-mouse horseradish peroxidase linked, 1/5000 dilution