膜Integrity WB抗体Cocktail (ab110414)

Abreviews (1)Q&A (8)Specific References (6)
Customer reviews and FAQs

概述

  • 产品名称膜Integrity WB抗体Cocktail
  • 检测类型Quantitative
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 产品概述

    ab110414 (MS620) contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:


     


    Outer Membrane - Porin ab14734 (MSA03)


    Intermembrane Space - Cytochrome c ab110325 (MSA06)


    Inner Membrane - Complex Va ab110273 (MS502) and Complex III Core 1 ab110252 (MS303)


    Matrix Space - Cyclophilin D ab110324 (MSA04)


     


    This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Va, 49kDa Complex III Core 1, and 21kDa Cyclophilin D. Human heart mitochondria are available as standard control (ab110337) mitochondria from other species and tissues are available.


    Cocktail Antibodies:


    Mouse monoclonal [20B12AF2] to VDAC1 / Porin - Mitochondrial Loading Control (ab14734):
    Amount: 50ug
    Working concentration: 2ug/ml


    Mouse monoclonal [E11AE12BD4] to Cyclophilin 40 (ab110324):
    Amount: 50ug
    Working concentration: 2ug/ml


    Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I (ab110252):
    Amount: 50ug
    Working concentration: 2ug/ml


    Mouse monoclonal [37BA11] to Cytochrome C (ab110325):
    Amount: 5ug
    Working concentration: 0.1ug/ml


    Mouse monoclonal [7H10BD4F9] to ATP5A (ab110273):
    Amount: 100ug
    Working concentration: 4ug/ml

  • 经测试应用适用于: WBmore details

性能

    应用

    Our Abpromise guarantee covers the use of ab110414 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    应用 Ab评论 说明
    WB Use a concentration of 10.2 µg/ml. The antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of10.2 µg/mL for Western blotting.

    Membrane Integrity WB Antibody Cocktail 图像

    • Western blot - MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414)
      Western blot - MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414)
      All lanes : Membrane Integrity WB Antibody Cocktail (ab110414)

      Lane 1 : Rat liver mitos prep #1
      Lane 2 : Rat liver mitos prep #2

      Lysates/proteins at 10 µg per lane.

    Membrane Integrity WB Antibody Cocktail (ab110414)参考文献

    This product has been referenced in:
    • Melis N  et al. Targeting eIF5A Hypusination Prevents Anoxic Cell Death through Mitochondrial Silencing and Improves Kidney Transplant Outcome. J Am Soc Nephrol N/A:N/A (2016). Read more (PubMed: 27612998) »
    • Baudot AD  et al. Using enhanced-mitophagy to measure autophagic flux. Methods 75:105-11 (2015). WB . Read more (PubMed: 25498004) »

    See all 6 Publications for this product

    发表研究结果有使用 ab110414？请让我们知道，以便我们可以引用本数据表中的参考文章。

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    Western Blot abreview for MitoProfile® Membrane Integrity WB Antibody Cocktail

     Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Application: Western Blot
    Sample: Mouse whole cell lysate (3T3 SA cell line)
    Loading amount: 20ug
    Gel Running conditions: reduced denaturating (4-12% gradient gel)
    Blocking step: 30 min in 5% milk at room temperature
    Dilution: 1/1000
    Incubation time: over night at 4°C or 1 hour at room temperature
    Secondary antibody: non Abcam goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
    Detection: ECL
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    Abcam user community

    Verified customer

    提交于 Aug 21 2014

    Hello

    I am enquiring into the mitosciences Sucrose gradient separation protocol.

    I am keep to perform this and attempt to confirm my protein is mitochondrial, however have only got access to fixed angle rotor for the ultracentrifu...

    Read More

    Thank you for your enquiry.

    MitoSciences Sucrose gradient (ab110414) protocol is a protein subfractionation method optimized for mitochondria. Therefore, the starting material is enriched mitochondria.

    To fractionation mitochondrial...

    Read More

    Hola,
    Envío adjunto un power point con la imagen de un western realizado con el kit MitoProfile® Membrane Integrity WB Antibody Cocktail en extractos mitocondriales de corazón humano.
    Como describe el folleto del kit aparece...

    Read More

    Gracias por contactarnos.

    Atendiendo a la imagen enviada, las bandas parecen corresponder con las esperadas correspondientes a las distintas estructuras mitocondriales.

    Es muy posible que la banda más intensa que sale entre 5...

    Read More

    Thank you for your fast response, I have some specific questions:

    1. Does the MitoProfile; Membrane Integrity WB Antibody Cocktail (ab110414) work for whole cell extracts?

    2. Do the antibodies come in a tube or separate tubes?

    Read More

    Thank for your reply.

    1. The MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414) should be suitable for whole cell extracts. You may observe some background staining as the product has been optimized on Mitochondrial extracts, an...

    Read More

    ich hoffe, ich habe Ihren Namen richtig verstanden. Wie gestern telefonisch besprochen, finden Sie unten eine Zusammenfassung unseres Projektes mit der Bitte um Weiterleitung an die Spezialisten bei MitoSciences. Über eine möglichst rascher R...

    Read More

    Vielen Dank für die Zusammenfassung in Englisch. Ich habe nun eine Antwort von unseren Mitochondrien -Spezialisten aus dem Labor erhalten.

    Ich habe die Antwort nicht übersetzt um Übersetzungsfehler zu vermeiden:

    "...

    Read More

    We need to know if in performing in w. blot this antibody cocktail the protocol is the same as per one single antibody.
    Thanks in advance and regards

    Thank you for your inquiry.


    Yes, you use a cocktail antibody in the same way as a regular antibody in WB. You'll need an anti-mouse IgGHRP conjugated antibody. You can load 10ug of the rat liver mitochondria as a control.

    Read More

    How much is recommended to use of the positive control per gel/experiment?

    Thank you for your inquiry. I am happy to confirm the amounts of positive control per gel: Please use 10ug of the rat liver prep each lane or any positive control that is given. I hope this information is helpful and wish you a good start into the new ...

    Read More

    Thank you very much for your reply, I forwarded the mail to the customer. She now explains her project and wants me to ask you if you have any ideas, or products which she might be able to use.   Here is her mail:   Thanks so much for finding ou...

    Read More

    Thank you for your reply. I have been in touch with our colleagues at Mitosciences who have kindly provided the following information: To answer your question directly, The antibody coating the plate supplied with Kit MTOX1 ab109903 does not cr...

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    I have been isolating some mitochondria from various rat tissues (Kidney, liver, brain and heart) and was thinking of using your antibody cocktail to demonstrate mitochondrial integrity (or lack of) via western blot. I was wondering if you could maybe ...

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    Thank you for contacting us with your questions about ab110414. The antibodies will be provided in one tube, at a total concentration of 2.37 mg/mL. The individual amounts of each antibody are available on the datasheet. We recommend using the cocktail...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"