膜Integrity WB抗体Cocktail (ab110414)

Abreviews (1)Q&A (8)Specific References (8)

概述

  • 产品名称
    膜Integrity WB抗体Cocktail
  • 检测类型
    Quantitative
  • 种属反应性
    与反应: Mouse, Rat, Cow, Human
  • 产品概述

    ab110414 (MS620) contains 5 mAbs against representative proteins for each of the primary structures of the mitochondrion:


    Outer Membrane - Porin ab14734 (MSA03)
    Intermembrane Space - Cytochrome c ab110325 (MSA06)
    Inner Membrane - Complex Va ab110273 (MS502) and Complex III Core 1 ab110252 (MS303)
    Matrix Space - Cyclophilin D ab110324 (MSA04)


    This kit is useful in Western blotting to evaluate the structural integrity of isolated mitochondria. This kit is reactive against 39kDa human porin, 12kDa cytochrome c, 55kDa Complex Va, 49kDa Complex III Core 1, and 21kDa Cyclophilin D. 


    Cocktail Antibodies:



    • Mouse monoclonal [20B12AF2] to VDAC1 / Porin - Mitochondrial Loading Control (ab14734):
      Amount: 50µg
      Working concentration: 2µg/ml

    • Mouse monoclonal [E11AE12BD4] to Cyclophilin 40 (ab110324):
      Amount: 50µg
      Working concentration: 2µg/ml

    • Mouse monoclonal [16D10AD9AH5] to Ubiquinol-Cytochrome C Reductase Core Protein I (ab110252):
      Amount: 50µg
      Working concentration: 2µg/ml

    • Mouse monoclonal [37BA11] to Cytochrome C (ab110325):
      Amount: 5µg
      Working concentration: 0.1µg/ml

    • Mouse monoclonal [7H10BD4F9] to ATP5A (ab110273):
      Amount: 100µg
      Working concentration: 4µg/ml

  • 经测试应用
    适用于: WBmore details

性能

应用

Our Abpromise guarantee covers the use of ab110414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 10.2 µg/ml. The antibody cocktail (2.37 mg/mL) should be diluted to a final working concentration of10.2 µg/mL for Western blotting.

图片

  • Western blot - MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414)
    Western blot - MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414)
    All lanes : Membrane Integrity WB Antibody Cocktail (ab110414)

    Lane 1 : Rat liver mitos prep #1
    Lane 2 : Rat liver mitos prep #2

    Lysates/proteins at 10 µg per lane.
  • Western blot - MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414)
    Western blot - MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414)This image is courtesy of an anonymous Abreview

    Sample: Mouse whole cell lysate (3T3 SA cell line)
    Loading amount: 20µg
    Gel Running conditions: reduced denaturating (4-12% gradient gel)
    Blocking step: 30 min in 5% milk at room temperature
    Dilution: 1/1000
    Incubation time: Overnight at 4°C or 1 hour at room temperature
    Secondary antibody: Goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
    Detection: ECL

文献

This product has been referenced in:
  • Sakamaki JI  et al. Bromodomain Protein BRD4 Is a Transcriptional Repressor of Autophagy and Lysosomal Function. Mol Cell 66:517-532.e9 (2017). Read more (PubMed: 28525743) »
  • Horibata Y  et al. Identification of the N-terminal transmembrane domain of StarD7 and its importance for mitochondrial outer membrane localization and phosphatidylcholine transfer. Sci Rep 7:8793 (2017). Read more (PubMed: 28821867) »

See all 8 Publications for this product

发表研究结果有使用 ab110414？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

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9 reviews or Q&A

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Western Blot abreview for MitoProfile® Membrane Integrity WB Antibody Cocktail

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
Application: Western Blot
Sample: Mouse whole cell lysate (3T3 SA cell line)
Loading amount: 20ug
Gel Running conditions: reduced denaturating (4-12% gradient gel)
Blocking step: 30 min in 5% milk at room temperature
Dilution: 1/1000
Incubation time: over night at 4°C or 1 hour at room temperature
Secondary antibody: non Abcam goat anti-mouse horseradish peroxidase linked, 1/5000 dilution
Detection: ECL
abreview image
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Abcam user community

Verified customer

提交于 Aug 21 2014

Hello

I am enquiring into the mitosciences Sucrose gradient separation protocol.

I am keep to perform this and attempt to confirm my protein is mitochondrial, however have only got access to fixed angle rotor for the ultracentrifu...

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Thank you for your enquiry.

MitoSciences Sucrose gradient (ab110414) protocol is a protein subfractionation method optimized for mitochondria. Therefore, the starting material is enriched mitochondria.

To fractionation mitochondrial...

Read More

Hola,
Envío adjunto un power point con la imagen de un western realizado con el kit MitoProfile® Membrane Integrity WB Antibody Cocktail en extractos mitocondriales de corazón humano.
Como describe el folleto del kit aparece...

Read More

Gracias por contactarnos.

Atendiendo a la imagen enviada, las bandas parecen corresponder con las esperadas correspondientes a las distintas estructuras mitocondriales.

Es muy posible que la banda más intensa que sale entre 5...

Read More

Thank you for your fast response, I have some specific questions:

1. Does the MitoProfile; Membrane Integrity WB Antibody Cocktail (ab110414) work for whole cell extracts?

2. Do the antibodies come in a tube or separate tubes?

Read More

Thank for your reply.

1. The MitoProfile® Membrane Integrity WB Antibody Cocktail (ab110414) should be suitable for whole cell extracts. You may observe some background staining as the product has been optimized on Mitochondrial extracts, an...

Read More

ich hoffe, ich habe Ihren Namen richtig verstanden. Wie gestern telefonisch besprochen, finden Sie unten eine Zusammenfassung unseres Projektes mit der Bitte um Weiterleitung an die Spezialisten bei MitoSciences. Über eine möglichst rascher R...

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Vielen Dank für die Zusammenfassung in Englisch. Ich habe nun eine Antwort von unseren Mitochondrien -Spezialisten aus dem Labor erhalten.

Ich habe die Antwort nicht übersetzt um Übersetzungsfehler zu vermeiden:

"...

Read More

We need to know if in performing in w. blot this antibody cocktail the protocol is the same as per one single antibody.
Thanks in advance and regards

Thank you for your inquiry.


Yes, you use a cocktail antibody in the same way as a regular antibody in WB. You'll need an anti-mouse IgGHRP conjugated antibody. You can load 10ug of the rat liver mitochondria as a control.

Read More

How much is recommended to use of the positive control per gel/experiment?

Thank you for your inquiry. I am happy to confirm the amounts of positive control per gel: Please use 10ug of the rat liver prep each lane or any positive control that is given. I hope this information is helpful and wish you a good start into the new ...

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Thank you very much for your reply, I forwarded the mail to the customer. She now explains her project and wants me to ask you if you have any ideas, or products which she might be able to use.   Here is her mail:   Thanks so much for finding ou...

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Thank you for your reply. I have been in touch with our colleagues at Mitosciences who have kindly provided the following information: To answer your question directly, The antibody coating the plate supplied with Kit MTOX1 ab109903 does not cr...

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I have been isolating some mitochondria from various rat tissues (Kidney, liver, brain and heart) and was thinking of using your antibody cocktail to demonstrate mitochondrial integrity (or lack of) via western blot. I was wondering if you could maybe ...

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Thank you for contacting us with your questions about ab110414. The antibodies will be provided in one tube, at a total concentration of 2.37 mg/mL. The individual amounts of each antibody are available on the datasheet. We recommend using the cocktail...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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