概述

  • 产品名称Anti-Melphalan抗体[Amp4/42]
  • 描述
    小鼠单克隆抗体[Amp4/42] to Melphalan
  • 特异性This antibody recognises the ring-opened structure that forms when DNA is alkylated by the anti-cancer drug melphalan and is then exposed to alkali. The alkali causes guanine bases alkylated at the N7 position to undergo a so-called ring-opening reaction and this antibody appears to recognise this structure, although recognition may be influenced by the neighbouring DNA sequence. The products of the ring-opening reaction are more resistant to depurination reactions than the original guanine adducts. This antibody was developed to improve detection of melphalan-DNA adducts through its ability to recognise the stabilised adduct. The Amp4/42 clone did not bind to alkali-treated control DNA or to DNA that had been alkylated with melphalan but not exposed to alkali. This antibody can be used for quantification of melphalan adducts in purified DNA by ELISA and for immunofluorescent detection of melphalan-DNA adducts in single cells.
  • 经测试应用适用于: ELISAmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    DNA/ melphalan alkylated DNA.

  • 阳性对照
    • DNA/ melphalan alkylated DNA.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • 纯度IgG fraction
  • 克隆单克隆
  • 克隆编号Amp4/42
  • 骨髓瘤Y3/Ag1.2.3
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab97554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use at an assay dependent dilution.

Anti-Melphalan antibody [Amp4/42] (ab97554)参考文献

This product has been referenced in:
  • Rothbarth J  et al. Immunohistochemical detection of melphalan-DNA adducts in colon cancer cells in vitro and human colorectal liver tumours in vivo. Biochem Pharmacol 67:1771-8 (2004). Read more (PubMed: 15081876) »
  • Frank AJ & Tilby MJ Quantification of DNA adducts in individual cells by immunofluorescence: effects of variation in DNA conformation. Exp Cell Res 283:127-34 (2003). Read more (PubMed: 12581733) »

See all 5 Publications for this product

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