概述

  • 产品名称Anti-MELK抗体[EPR3981]
    参阅全部 MELK 一抗
  • 描述
    兔单克隆抗体[EPR3981] to MELK
  • 经测试应用适用于: WBmore details
    不适用于: Flow Cyt,ICC,IHC-P or IP
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MELK (N terminal). The exact sequence is proprietary.

  • 阳性对照
    • WB: K562, 293T, and Jurkat cell lysates.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab108529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Predicted molecular weight: 75 kDa.
  • 应用说明Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • 靶标

    • 功能Phosphorylates ZNF622 and may contribute to its redirection to the nucleus. May be involved in the inhibition of spliceosome assembly during mitosis.
    • 组织特异性Expressed in placenta, kidney, thymus, testis, ovary and intestine.
    • 序列相似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
      Contains 1 KA1 (kinase-associated) domain.
      Contains 1 protein kinase domain.
    • 翻译后修饰Autophosphorylated. Thr-478 phosphorylation during mitosis promotes interaction with PPP1R8.
    • 细胞定位Cytoplasm.
    • Information by UniProt
    • 数据库链接
    • 别名
      • AI327312 antibody
      • hMELK antibody
      • HPK 38 antibody
      • hPK38 antibody
      • KIAA0175 antibody
      • Likely ortholog of maternal embryonic leucine zipper kinase antibody
      • Maternal embryonic leucine zipper kinase antibody
      • MELK antibody
      • MELK_HUMAN antibody
      • mKIAA0175 antibody
      • MPK38 antibody
      • OTTHUMP00000021377 antibody
      • OTTHUMP00000046113 antibody
      • pEg3 kinase antibody
      • Protein kinase Eg3 antibody
      • Protein kinase PK38 antibody
      • RP23 382O11.1 antibody
      • Tyrosine protein kinase MELK antibody
      see all

    Anti-MELK antibody [EPR3981] 图像



    • Predicted band size : 75 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MELK knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: HCT116 cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab108529 observed at 75 kDa. Red - loading control, ab8226, observed at 42 kDa.

      ab108529 was shown to recognize MELK when MELK knockout samples were used, along with additional cross-reactive bands. Wild-type and MELK knockout samples were subjected to SDS-PAGE. ab108529 and ab8226 (loading control to beta Actin) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) ab216776 secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-MELK antibody [EPR3981] (ab108529) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 75 kDa

    Anti-MELK antibody [EPR3981] (ab108529)参考文献

    This product has been referenced in:
    • Wang Y  et al. Mitotic MELK-eIF4B signaling controls protein synthesis and tumor cell survival. Proc Natl Acad Sci U S A 113:9810-5 (2016). Read more (PubMed: 27528663) »
    • Wang Y  et al. MELK is an oncogenic kinase essential for mitotic progression in basal-like breast cancer cells. Elife 3:e01763 (2014). Human . Read more (PubMed: 24844244) »

    See all 3 Publications for this product

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