概述

  • 产品名称
    Anti-Melanopsin抗体
    参阅全部 Melanopsin 一抗
  • 描述
    兔多克隆抗体to Melanopsin
  • 经测试应用
    适用于: ICC/IF, IHC-Fr, IHC-P, WBmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Rat Melanopsin aa 455-474.
    Sequence:

    EQKSKTPKTKRHLPSLDRRM

  • 阳性对照
    • Rat retinal ganglion cells (RGCs) for immunofluorescence and immunohistochemistry.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab19383 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 5 µg/ml.
IHC-Fr 1/1000.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.1 - 0.2 µg/ml. Detects a band of approximately 53,85 kDa. Not recommended for the detection of endogenously expressed protein.

靶标

图片

  • Immunofluorescence staining of melanopsin in rat retinal ganglion cells using ab19383. 

文献

This product has been referenced in:
  • Diessler S  et al. Rai1 frees mice from the repression of active wake behaviors by light. Elife 6:N/A (2017). Read more (PubMed: 28548639) »
  • Man PS  et al. Rhythmic expression of an egr-1 transgene in rats distinguishes two populations of photoreceptor cells in the retinal outer nuclear layer. Mol Vis 14:1176-86 (2008). IHC . Read more (PubMed: 18587494) »

See all 2 Publications for this product

客户评价及客户问答

Thank you for your enquiry. Further to your technical enquiry I have been in touch with my colleague who removed mouse reactivity for this antibody. Apparently the customer who complained about the murine reactivity used the antibody up to concentr...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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