Melanophilin overexpression 293T裂解物(whole cell) (ab94249)
概述
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产品名称
Melanophilin overexpression 293T裂解物(whole cell) -
常规说明
ab94249 is a 293T cell transfected lysate in which Human Melanophilin has been transiently over-expressed using a pCMV-Melanophilin plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A. Disease: Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations. Similarity: Contains 1 FYVE-type zinc finger. Contains 1 RabBD (Rab-binding) domain.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94249于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94249 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-Melanophilin antibody (ab89169) at 1/500 dilution
Lane 1 :Melanophilin overexpression 293T lysate (whole cell) (ab94249)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94249 尚未被引用在任何文献中。