概述

  • 产品名称
    Anti-Melanophilin抗体
    参阅全部 Melanophilin 一抗
  • 描述
    山羊多克隆抗体to Melanophilin
  • 特异性
    ab2716 recognises Melanophilin.
  • 经测试应用
    适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    ARHIFAKPVMAQQP

    , corresponding to C terminal amino acids 577-590 of Mouse Melanophilin

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 纯化说明
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab2716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能
    Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
  • 疾病相关
    Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations.
  • 序列相似性
    Contains 1 FYVE-type zinc finger.
    Contains 1 RabBD (Rab-binding) domain.
  • 细胞定位
    Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2210418F23Rik antibody
    • 5031433I09Rik antibody
    • AW228792 antibody
    • D1Wsu84e antibody
    • Exophilin 3 antibody
    • Exophilin-3 antibody
    • l(1)-3Rk antibody
    • l1Rk3 antibody
    • Leaden antibody
    • Leaden protein antibody
    • ln antibody
    • Melanophilin antibody
    • MELPH_HUMAN antibody
    • MGC2771 antibody
    • MGC59733 antibody
    • Mlph antibody
    • Slac 2a antibody
    • SlaC2-a antibody
    • Slp homolog lacking C2 domains a antibody
    • Synaptotagmin like protein 2a antibody
    • Synaptotagmin like protein lacking C2 domains A antibody
    • Synaptotagmin-like protein 2a antibody
    see all

图片

  • All lanes : Anti-Melanophilin antibody (ab2716) at 0.5 µg/ml

    Lane 1 : total melanocyte PNS (200ug per lane) of wild-type
    Lane 2 : total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice


    Predicted band size : 65 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)
    ab2716 staining (3µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hours.  Detected by western blot using chemiluminescence.
  • Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.

文献

This product has been referenced in:
  • Kudo M  et al. Bifunctional effects of O-methylated flavones from Scutellaria baicalensis Georgi on melanocytes: Inhibition of melanin production and intracellular melanosome transport. PLoS One 12:e0171513 (2017). Read more (PubMed: 28182699) »

See 1 Publication for this product

客户评价及客户问答

This antibody has not yet been tested for use in IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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