Anti-Melanophilin抗体(ab2716)

概述

  • 产品名称Anti-Melanophilin抗体
    参阅全部 Melanophilin 一抗
  • 描述
    山羊多克隆抗体to Melanophilin
  • 特异性ab2716 recognises Melanophilin.
  • 经测试应用适用于: WB, ELISA, ICC/IFmore details
  • 种属反应性
    与反应: Mouse
    预测可用于: Rat
  • 免疫原

    Synthetic peptide:

    ARHIFAKPVMAQQP

    , corresponding to C terminal amino acids 577-590 of Mouse Melanophilin

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 纯化说明Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab2716 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 65 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

靶标

  • 功能Rab effector protein involved in melanosome transport. Serves as link between melanosome-bound RAB27A and the motor protein MYO5A.
  • 疾病相关Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3) [MIM:609227]. GS3 is a rare autosomal recessive disorder characterized by pigmentary dilution of the skin and hair, the presence of large clumps of pigment in hair shafts, and an accumulation of melanosomes in melanocytes, without other clinical manifestations.
  • 序列相似性Contains 1 FYVE-type zinc finger.
    Contains 1 RabBD (Rab-binding) domain.
  • 细胞定位Cytoplasm.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 2210418F23Rik antibody
    • 5031433I09Rik antibody
    • AW228792 antibody
    • D1Wsu84e antibody
    • Exophilin 3 antibody
    • Exophilin-3 antibody
    • l(1)-3Rk antibody
    • l1Rk3 antibody
    • Leaden antibody
    • Leaden protein antibody
    • ln antibody
    • Melanophilin antibody
    • MELPH_HUMAN antibody
    • MGC2771 antibody
    • MGC59733 antibody
    • Mlph antibody
    • Slac 2a antibody
    • SlaC2-a antibody
    • Slp homolog lacking C2 domains a antibody
    • Synaptotagmin like protein 2a antibody
    • Synaptotagmin like protein lacking C2 domains A antibody
    • Synaptotagmin-like protein 2a antibody
    see all

Anti-Melanophilin antibody 图像

  • All lanes : Anti-Melanophilin antibody (ab2716) at 0.5 µg/ml

    Lane 1 : total melanocyte PNS (200ug per lane) of wild-type
    Lane 2 : total melanocyte PNS (200ug per lane) of leaden (Mlph null) mice


    Predicted band size : 65 kDa
    Observed band size : 81 kDa (why is the actual band size different from the predicted?)
    ab2716 staining (3µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 12 hours.  Detected by western blot using chemiluminescence.
  • Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.Wild-type C57bl6 derived melanocytes were stained with ab2716 (0.5ug/ml) and detected with Alexa568 conjugated donkey anti-sheep second antibodies. Brightfield image shows melanosome distribution. Data kindly provided by A N Hume, Imperial College.

Anti-Melanophilin antibody (ab2716)参考文献

ab2716 has not yet been referenced specifically in any publications.

Product Wall

This antibody has not yet been tested for use in IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"