The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明ELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
sELISA: Use at a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 – 0.4 ng/well of recombinant human protein.
WB: Use at a concentration of 0.1 - 0.2 µg/ml. Detection limit for recombinant human protein is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Predicted molecular weight: 15 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
功能Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.
组织特异性All malignant melanoma cell lines tested and infrequently in glioma cell lines.
序列相似性Belongs to the MIA/OTOR family. Contains 1 SH3 domain.
翻译后修饰May possess two intramolecular disulfide bonds.
Flow Cytometry - Anti-Melanoma Inhibitory Activity antibody (Biotin) (ab84235)This image is courtesy of an anonymous Abreview
ab84235 staining Melanoma Inhibitory Activity in the Human melanoma cell line by Flow Cytometry. Cells were fixed with formaldehyde and permeabilized with saponine. The sample was incubated with the primary antibody (1/100 in PBS) for 15 minutes at 20°C. ab97050, Goat anti-rabbit IgG polyclonal (1/100) was used as the secondary antibody.