Anti-Melanoma gp100抗体(ab52058)

概述

  • 产品名称Anti-Melanoma gp100抗体
    参阅全部 Melanoma gp100 一抗
  • 描述
    山羊多克隆抗体to Melanoma gp100
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Dog, Pig
  • 免疫原

    Synthetic peptide:

    CPIGENSPLLSGQQ

    , corresponding to C terminal amino acids 647-660 of Human Melanoma gp100

  • 阳性对照
    • Human skin lysate

性能

应用

Our Abpromise guarantee covers the use of ab52058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明Peptide ELISA: Antibody detection limit dilution 1:16,000.
    WB: Use at a concentration of 1.0 - 3.0 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 70 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.
    • 组织特异性Preferentially expressed in melanomas. Some expression was found in dysplastic nevi. Not found in normal tissues nor in carcinomas. Normally expressed at low levels in quiescent adult melanocytes but overexpressed by proliferating neonatal melanocytes and during tumor growth.
    • 序列相似性Belongs to the PMEL/NMB family.
      Contains 1 PKD domain.
    • 结构域The RPT domain is essential for the generation of the fibrillar matrix of melanosomes.
      The lumenal domain is necessary for correct processing and trafficking to melanosomes.
    • 翻译后修饰A small amount of P1/P100 (major form) undergoes glycosylation to yield P2/P120 (minor form). P2 is cleaved by a furin-like proprotein convertase (PC) in a pH-dependent manner in a post-Golgi, prelysosomal compartment into two disulfide-linked subunits: a large lumenal subunit, M-alpha/ME20-S, and an integral membrane subunit, M-beta. Despite cleavage, only a small fraction of M-alpha is secreted, whereas most M-alpha and M-beta remain associated with each other intracellularly. M-alpha is further processed to M-alpha N and M-alpha C. M-alpha C further undergoes processing to yield M-alpha C1 and M-alpha C3 (M-alpha C2 in the case of PMEL17-is or PMEL17-ls). Formation of intralumenal fibrils in the melanosomes requires the formation of M-alpha that becomes incorporated into the fibrils. Stage II melanosomes harbor only Golgi-modified Pmel17 fragments that are derived from M-alpha and that bear sialylated O-linked oligosaccharides.
      N-glycosylated. O-glycosylated; contains sialic acid.
    • 细胞定位Secreted and Endoplasmic reticulum membrane. Golgi apparatus. Melanosome. Endosome > multivesicular body. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Localizes predominantly to intralumenal vesicles (ILVs) within multivesicular bodies. Associates with ILVs found within the lumen of premelanosomes and melanosomes and particularly in compartments that serve as precursors to the striated stage II premelanosomes.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 95 kDa melanocyte specific secreted glycoprotein antibody
      • 95 kDa melanocyte-specific secreted glycoprotein antibody
      • D12S53E antibody
      • gp100 antibody
      • M-beta antibody
      • ME20 antibody
      • ME20 M/ME20 S antibody
      • ME20-M antibody
      • ME20-S antibody
      • ME20M antibody
      • ME20M/ME20S antibody
      • ME20S antibody
      • Melanocyte lineage specific antigen GP100 antibody
      • Melanocyte protein mel 17 antibody
      • Melanocyte protein Pmel 17 antibody
      • Melanocyte protein Pmel 17 precursor antibody
      • Melanocytes lineage-specific antigen GP100 antibody
      • Melanoma associated ME20 antigen antibody
      • Melanoma gp100 antibody
      • Melanoma-associated ME20 antigen antibody
      • Melanosomal matrix protein 17 antibody
      • Melanosomal matrix protein17 antibody
      • P1 antibody
      • p100 antibody
      • p26 antibody
      • PMEL 17 antibody
      • PMEL antibody
      • PMEL_HUMAN antibody
      • PMEL17 antibody
      • Premelanosome protein antibody
      • Secreted melanoma-associated ME20 antigen antibody
      • SI antibody
      • SIL antibody
      • SILV antibody
      • Silver (mouse homolog) like antibody
      • Silver homolog antibody
      • Silver locus protein homolog antibody
      • Silver, mouse, homolog of antibody
      see all

    Anti-Melanoma gp100 antibody 图像

    • Anti-Melanoma gp100 antibody (ab52058) at 1 µg/ml + Human Skin lysate at 35 µg

      Predicted band size : 70 kDa
      Additional bands at : 26 kDa (possible immature (unprocessed)).

      Primary incubation was 1 hour. Detected by chemiluminescence. The 26kDa band observed in Human Skin lysate corresponds to the precusor protein M-beta fragment (Leonhardt et al, Mol Biol Cell. 2013 Apr;24(7):964-81.PMID: 23389629).

    • All lanes : Anti-Melanoma gp100 antibody (ab52058) at 1 µg/ml

      Lane 1 : Cell lysates prepared from mock transfected HEK293 cells
      Lane 2 : Cell lysates prepared from melanoma gp100 transfected HEK293 cells


      Predicted band size : 70 kDa

    Anti-Melanoma gp100 antibody (ab52058)参考文献

    This product has been referenced in:
    • Grifoni SD  et al. Hsc70 regulates cell surface ASIC2 expression and vascular smooth muscle cell migration. Am J Physiol Heart Circ Physiol : (2008). Read more (PubMed: 18310515) »

    See 1 Publication for this product

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    Thank you for your inquiry.

    I can confirm that it is possible to assay proteins via ELISA.

    We have the following products that will be suitable for gp100:

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    Thank you for your enquiry and your interest in our products.

    I regret to inform you that the immunogen used to raise this antibody is not commercially available so it is not in our catalogue.

    Apologies!

    If you need any fu...

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    Application Western blot
    Sample Mouse Cell lysate - whole cell (HEK293 transfected with mouse GP100 plasmid)
    Loading amount 10000 cells
    Specification HEK293 transfected with mouse GP100 plasmid
    Treatment 44 hrs post transfection
    Gel Running Conditions Reduced Denaturing (4-12% Bis Tris)
    Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
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    提交于 Sep 29 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"