概述

  • 产品名称Anti-Melanoma抗体[PNL2]
    参阅全部 Melanoma 一抗
  • 描述
    小鼠单克隆抗体[PNL2] to Melanoma
  • 特异性This antibody labels melanocytes in the cytoplasm and plasma membrane and is useful tool for the identification of melanomas and clear cell sarcomas. It seems to be a melanocyte antibody of autoimmune origin as it does not react with the non-melanocytic peptide antigen used for immunization.
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide.

  • 阳性对照
    • Melanoma.

性能

应用

Our Abpromise guarantee covers the use of ab12502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval with 1 mM EDTA buffer, pH 9.0 before commencing with IHC staining protocol. We suggest an incubation period of 30-60 minutes at room temperature. This antibody may be diluted to a titer of 1:10-1:25 in an ABC method.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
    • 细胞定位Membrane; Single-pass membrane protein
    • 数据库链接
    • 别名
      • Antigen LB39 AA antibody
      • Antigen SK29 AA antibody
      • MART1 antibody
      • melan A antibody
      • Melan A protein antibody
      • Melanoma antigen recognized by T-cells 1 antibody
      • Monophenol monooxygenase antibody
      • Tumor rejection antigen AB antibody
      • tyrosinase antibody
      see all

    Anti-Melanoma antibody [PNL2] 图像

    • IHC image of ab12502 staining in human melanoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab12502, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Anti-Melanoma antibody [PNL2] (ab12502)参考文献

    This product has been referenced in:
    • Rochaix P  et al. PNL2, a new monoclonal antibody directed against a fixative-resistant melanocyte antigen. Mod Pathol 16:481-90 (2003). Read more (PubMed: 12748255) »

    See 1 Publication for this product

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