Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3] (ab732)

概述

  • 产品名称
    Anti-Melanoma抗体[HMB45 + M2-7C10 + M2-9E3]
    参阅全部 Melanoma 一抗
  • 描述
    小鼠单克隆抗体[HMB45 + M2-7C10 + M2-9E3] to Melanoma
  • 经测试应用
    适用于: Flow Cyt, IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat
  • 免疫原

    HMB45 - Pigmented melanoma metastases from LN MART-1 - Recombinant human MART-1 protein

  • 表位
    HMB45 and MART-1
  • 阳性对照
    • Metastatic melanoma in lymph node.
  • 常规说明

    Melanoma Cocktail is HMB45[HMB45] + two different clones of MART-1[M2-7C10] + [M2-9E3]. HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b

    HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones M2-7C10 and M2-9E3. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.

    This product was changed from ascites to tissue culture supernatant on [14/07/17]. The following lots are from ascites and are still in stock as of [14/07/17] – [GR313023, GR3175007]. Lot numbers higher than [GR313023] will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.099% Sodium azide
    Constituents: 0.9% Proprietary component, 99% Water
  • 纯度
    Tissue culture supernatant
  • Primary antibody说明
    HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Thus, the HMB-45 and MART-1 cocktail is potentially more sensitive than HMB-45 or MART-1 alone. MART-1 is a cocktail of clones DT101 and BC199. The combination of HMB-45 and the MART-1 cocktail make this triple antibody cocktail a first-order pan melanoma screener.
  • 克隆
    单克隆
  • 克隆编号
    HMB45 + M2-7C10 + M2-9E3
  • 骨髓瘤
    unknown
  • 同种型
    IgG2b
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use at an assay dependent concentration. PubMed: 22147606ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-P 1/100 - 1/200.

The staining intensity of this melanoma cocktail is enhanced by performing heat mediated antigen retrieval using a high pH buffer. The HMB-45 portion of this antibody will stain with citrate buffer, pH 6.0; however, data shows the MART-1 portion of this cocktail may be negative unless a high pH buffer is used. We therefore, strongly recommend that you do not use citrate buffer for this antibody cocktail.

IHC-Fr Use at an assay dependent concentration.

靶标

图片

  • ab732 staining Melanoma in Human lymph node tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections).

  • ab732 staining murine melanoma metastasis tissue sections by IHC-P.  Tissue sections were paraformaldehyde fixed and blocked with 0.5% Perkin-Elmer TNB Blocking Buffer for 30 minutes at 25°C.  The primary antibody was diluted 1/50 and incubated for 18 hours at 4°C.  An Alexa Fluor 488® conjugated goat anti-mouse was used as the secondary

    Confocal image shows melanoma in magenta, vessels in red (Collagen Type IV, ab19808), and melanin in cyan. Tissue is perfusion-fixed 15µm cryostat sections mounted on slides (i.e., lightly fixed, but not paraffin embedded).

    See Abreview

文献

This product has been referenced in:
  • Turajlic S  et al. Whole-genome sequencing reveals complex mechanisms of intrinsic resistance to BRAF inhibition. Ann Oncol 25:959-67 (2014). ICC/IF ; Human . Read more (PubMed: 24504448) »
  • Girotti MR  et al. Inhibiting EGF receptor or SRC family kinase signaling overcomes BRAF inhibitor resistance in melanoma. Cancer Discov 3:158-67 (2013). IHC ; Human . Read more (PubMed: 23242808) »

See all 13 Publications for this product

客户评价及客户问答

Thank you for contacting Abcam.
I have attached the Abcam, mouse on mouse staining protocol to see if that can give you any suggestions. If you can send me a copy of the protocol you are using, then I would be happy to see if there are any suggest...

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I am sorry to confirm we do not currently have fluorochrome conjugated Anti-Melanoma antibody [HMB45 + DT101 + BC199] (ab732) on our catalog.


However, you may like to consider either using a secondary antibody (http://www.abcam.com/index....

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Our Melanoma Cocktail is HMB45[HMB45] + two different clones of MART-1[M2-7C10] + [M2-9E3].
HMB34 isotype: IgG1/kappa and both clones of MART-1: IgG2b + IgG2b
Epitope/antigen: HMB45 and MART-1

Thank you very much for contacting us with your enquiry.
I am sorry to hear that there has been some variability with ab732. We don't have any other recent reports of the antibody not working, and there haven't been any changes to the antibody, so...

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Thank you for your enquiry. I am pleased to provide the following suggested positive controls for these antibodies. I have mainly chosen tissue that has been tested succesfully and shown in an image on the datasheets: 1.Anti-ErbB 2 antibody (ab2428) Hu...

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Thank you for your patience while I was looking into this. I have heard back from the lab and they have told me that ab732 was tested using the DIVA decloaking agent (http://biocare.net/wp-content/uploads/DV2004X.pdf) which is at pH.6.2. Also for the r...

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Thank you for contacting Abcam. The 3 clones in the ab732 cocktail do recognize mouse tissue. The IHCP protocol that was used to test the antibody was optimized to human tissue and the following protocol was used: Pretreatment Protocol Heat Retrieval M...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (murine melanoma metastasis)
Specification
murine melanoma metastasis
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
No
Blocking step
(agent) for 30 minute(s) · Concentration: 0.5% · Temperature: 25°C
Username

Abcam user community

Verified customer

提交于 Jan 07 2008

Thank you for your enquiry. You would not want to use the citrate buffer for antigen retrieval on your samples when using this antibody cocktail. You can use 1mM EDTA pH 8.0, and you should determine the optimal heating time for your sample. A goo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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