Anti-Melanoma抗体[Cocktail - HMB45+A103+T311], prediluted (ab82762)

概述

  • 产品名称
    Anti-Melanoma抗体[Cocktail - HMB45+A103+T311], prediluted
    参阅全部 Melanoma 一抗
  • 描述
    小鼠单克隆抗体[Cocktail - HMB45+A103+T311] to Melanoma, prediluted
  • 特异性
    This antibody has been shown to label the majority of melanomas.
  • 经测试应用
    适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Other Immunogen Type corresponding to Melanoma. Pigmented melanoma metastases from lymph nodes (HMB45). Recombinant human MART-1 protein (A103). Recombinant tyrosinase protein T311.

  • 阳性对照
    • Metastatic melanoma in lymph node.
  • 常规说明

    Isotypes: IgG1/kappa, IgG1 and IgG2a.
    The HMB45 clone reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45 reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. Clone A103 recognizes a protein of 20 kDa, identified as MART1 (melanoma antigen recognized by T cells 1) or MelanA. MelanA is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART1 and tyrosinase make this triple antibody cocktail a first order pan melanoma screener.

性能

  • 形式
    Prediluted
  • 存放说明
    Shipped at 4°C. Store at +4°C.
  • 存储溶液
    Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, Stabilizing agent, pH 7.5
  • Concentration information loading...
  • Primary antibody说明
    Isotypes: IgG1/kappa, IgG1 and IgG2a.
    The HMB45 clone reacts with a neuraminidase sensitive oligosaccharide side chain of a glycoconjugate present in immature melanosomes. The HMB45 reactive antigen is present in cutaneous melanocytes, prenatal and infantile retinal pigment epithelium and melanoma cells and is thought to be oncofetal in nature. Clone A103 recognizes a protein of 20 kDa, identified as MART1 (melanoma antigen recognized by T cells 1) or MelanA. MelanA is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Studies have also shown that MART1 is more sensitive than HMB45 when labeling metastatic melanomas. Tyrosinase is a key enzyme involved in the initial stages of melanin biosynthesis. Studies have shown tyrosinase to be a more sensitive marker when compared to HMB45 and MART1. It has been shown to label a higher percentage of desmoplastic melanomas than HMB45. The combination of HMB45, MART1 and tyrosinase make this triple antibody cocktail a first order pan melanoma screener.
  • 克隆
    单克隆
  • 克隆编号
    Cocktail - HMB45+A103+T311
  • 同种型
    IgG1
  • 轻链类型
    kappa
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab82762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/1.

靶标

  • 相关性
    Malignant melanoma is a malignant neoplasm of melanocytes, arising de novo or from a pre existing benign nevus, which occurs most often in the skin but also may involve other sites.
  • 细胞定位
    Membrane; Single-pass membrane protein
  • 数据库链接
  • 别名
    • Antigen LB39 AA antibody
    • Antigen SK29 AA antibody
    • MART1 antibody
    • melan A antibody
    • Melan A protein antibody
    • Melanoma antigen recognized by T-cells 1 antibody
    • Monophenol monooxygenase antibody
    • Tumor rejection antigen AB antibody
    • tyrosinase antibody
    see all

Anti-Melanoma antibody [Cocktail - HMB45+A103+T311], prediluted (ab82762)参考文献

This product has been referenced in:
  • Kapur RP  et al. Anti-melanoma monoclonal antibody HMB45 identifies an oncofetal glycoconjugate associated with immature melanosomes. J Histochem Cytochem 40:207-12 (1992). IHC-P, IHC-Fr . Read more (PubMed: 1552165) »
  • Gown AM  et al. Monoclonal antibodies specific for melanocytic tumors distinguish subpopulations of melanocytes. Am J Pathol 123:195-203 (1986). IHC-P, IHC-Fr . Read more (PubMed: 3518473) »

See all 2 Publications for this product

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