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RabMAb

Anti-MelanA抗体[EP1422Y] (ab51061)

概述

  • 产品名称
    Anti-MelanA抗体[EP1422Y]
    参阅全部 MelanA 一抗
  • 描述
    兔单克隆抗体[EP1422Y] to MelanA
  • 经测试应用
    适用于: WB, IHC-P, IHC-Fr, Flow Cytmore details
    不适用于: IP
  • 种属反应性
    与反应: Human
  • 免疫原

    corresponding to Human MelanA aa 1-100 (N terminal).
    Database link: Q16655

  • 阳性对照
    • Melanoma lysate or melanoma tissue.
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

性能

应用

Our Abpromise guarantee covers the use of ab51061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 20 kDa (predicted molecular weight: 13 kDa).
IHC-P 1/100 - 1/250.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明
    Is unsuitable for IP.
  • 靶标

    • 功能
      Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
    • 组织特异性
      Expression is restricted to melanoma and melanocyte cell lines and retina.
    • 翻译后修饰
      Acylated.
    • 细胞定位
      Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Antigen LB39 AA antibody
      • Antigen LB39-AA antibody
      • Antigen SK29 AA antibody
      • Antigen SK29-AA antibody
      • MAR1_HUMAN antibody
      • MART 1 antibody
      • MART-1 antibody
      • MART1 antibody
      • Melan A antibody
      • Melan A protein antibody
      • Melanoma antigen recognized by T cells 1 antibody
      • Melanoma antigen recognized by T-cells 1 antibody
      • MLAN A antibody
      • MLANA antibody
      • OTTHUMP00000021036 antibody
      • OTTHUMP00000021037 antibody
      • OTTHUMP00000021038 antibody
      • Protein Melan-A antibody
      see all

    Anti-MelanA antibody [EP1422Y] 图像

    • Ab51061 (1/100) staining human MelanA in melanoma tissue by immunohistochemistry using paraffin embedded tissue.
    • Immunohistochemical analysis of Human malignant melanoma, staining MelanA with ab51061 at 1/20000 dilution. A Cy3-conjugated donkey anti-rabbit IgG (1/500) was used as the secondary antibody.
    • Overlay histogram showing Malme-3M cells stained with ab51061 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51061, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-MelanA antibody [EP1422Y] (ab51061) at 1/1000 dilution + Melanoma lysate at 10 µg

      Secondary
      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 13 kDa
      Observed band size : 20 kDa (why is the actual band size different from the predicted?)
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    Anti-MelanA antibody [EP1422Y] (ab51061)参考文献

    This product has been referenced in:
    • Yanagita T  et al. Anti-SIRPa antibodies as a potential new tool for cancer immunotherapy. JCI Insight 2:e89140 (2017). Read more (PubMed: 28097229) »
    • Gao Y  et al. Enhancing the treatment effect on melanoma by heat shock protein 70-peptide complexes purified from human melanoma cell lines. Oncol Rep 36:1243-50 (2016). Human . Read more (PubMed: 27431432) »

    See all 11 Publications for this product

    Product Wall

    Merci de nous avoir contactés.

    Suite à notre conversation téléphonique, je me suis renseignée a propos de vos recherches. Apres réflexion, j’ai du mal à comprendre comment vous allez pouv...

    Read More

    Thank you for your enquiry.

    Indeed, the purification kits are designed for cleaning up small volumes of antibodies (ideallyin a volume of 100µl to 0.5ml), as you can see on the datasheet for ab102784.

    http://www.abcam.com/inde...

    Read More

    Thank you for contacting us.

    Having checked with the lab, I am sorry to let you know that we are unable to provide these MelanA antibodies in a buffer without BSA.

    However, if you need to remove BSA and other unwanted ingredients, I...

    Read More

    You are welcome! We are always happy to help :-) Have a great day!

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin, melanozytes, 10ym, OCT embedded)
    Specification
    skin, melanozytes, 10ym, OCT embedded
    Fixative
    Methanol
    Permeabilization
    No
    Username

    Dr. Christian Posch

    Verified customer

    提交于 Oct 21 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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