Anti-MelanA抗体[DT101 + BC199] (ab731)

Abreviews (2)Q&A (1)Specific References (10)

概述

  • 产品名称
    Anti-MelanA抗体[DT101 + BC199]
    参阅全部 MelanA 一抗
  • 描述
    小鼠单克隆抗体[DT101 + BC199] to MelanA
  • 宿主
    Mouse
  • 特异性
    This antibody recognizes a protein of 18kDa, identified at MART-1 (Melanoma Antigen Recognized by T- cells 1) or Melan-A. The MART-1 recognizes a subcellular fraction found in melanosomes. The antibody labels melanomas and tumors showing melanocytic differentiation. It does not mark neoplasms of epithelial origin, lymphomas or mesenchymal tumors. Melan-A is a useful addition to melanoma panels as it is apparently specific for melanocytic lesions. Both HMB-45 and MART-1 are coexpressed in the majority of melanomas, as well as uniquely expressed in certain cases. Studies have shown that MART-1 is more sensitive than HMB-45 when labeling metastatic melanomas.
  • 经测试应用
    适用于: IHC-P, IHC-Frmore details
  • 种属反应性
    与反应: Mouse, Rat, Dog, Human
  • 免疫原

    Recombinant full length protein (Human).

  • 阳性对照
    • Melanoma
  • 常规说明

    This antibody is a mixture of two clones, DT101 and BC199.

    This product was changed from ascites to tissue culture supernatant on 8th March 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: Proprietary, not sodium azide or thimerosal.
    Constituents: PBS, Tissue Culture Supernatant. pH 7.3; Protein carrier.
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    DT101 + BC199
  • 骨髓瘤
    unknown
  • 同种型
    IgG2b
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/50 - 1/100. ABC method.
It is sometimes difficult to interpret DAB stained melanomas due to endogenous pigment. We recommend you substitute an AEC or a Fast Red substrate protocol. An avidin biotin blocking may be required for certain types of melanomas.

靶标

  • 功能
    Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
  • 组织特异性
    Expression is restricted to melanoma and melanocyte cell lines and retina.
  • 翻译后修饰
    Acylated.
  • 细胞定位
    Endoplasmic reticulum membrane. Golgi apparatus. Golgi apparatus > trans-Golgi network membrane. Melanosome. Also found in small vesicles and tubules dispersed over the entire cytoplasm. A small fraction of the protein is inserted into the membrane in an inverted orientation. Inversion of membrane topology results in the relocalization of the protein from a predominant Golgi/post-Golgi area to the endoplasmic reticulum. Melanoma cells expressing the protein with an inverted membrane topology are more effectively recognized by specific cytolytic T-lymphocytes than those expressing the protein in its native membrane orientation.
  • Information by UniProt
  • 数据库链接
    see all
  • 别名
    • Antigen LB39 AA antibody
    • Antigen LB39-AA antibody
    • Antigen SK29 AA antibody
    • Antigen SK29-AA antibody
    • MAR1_HUMAN antibody
    • MART 1 antibody
    • MART-1 antibody
    • MART1 antibody
    • Melan A antibody
    • Melan A protein antibody
    • Melanoma antigen recognized by T cells 1 antibody
    • Melanoma antigen recognized by T-cells 1 antibody
    • MLAN A antibody
    • MLANA antibody
    • OTTHUMP00000021036 antibody
    • OTTHUMP00000021037 antibody
    • OTTHUMP00000021038 antibody
    • Protein Melan-A antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MelanA antibody [DT101 + BC199] (ab731)

    Immunohistochemistry analysis of Melanoma tissue labeling MelanA with ab731.

文献

This product has been referenced in:
  • Schwochow Thalmann D  et al. The evolution of Sex-linked barring alleles in chickens involves both regulatory and coding changes in CDKN2A. PLoS Genet 13:e1006665 (2017). IHC-Fr ; Chicken . Read more (PubMed: 28388616) »
  • Cao J  et al. Tuberous sclerosis complex inactivation disrupts melanogenesis via mTORC1 activation. J Clin Invest 127:349-364 (2017). Read more (PubMed: 27918305) »

See all 10 Publications for this product

发表研究结果有使用 ab731？请让我们知道，以便我们可以引用本数据表中的参考文章。

客户评价及客户问答

提交评价 提交问题

3 reviews or Q&A

10
Votes: Highest First

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [DT101 + BC199]

 Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 30 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer, pH6.0
Sample
Human Tissue sections (adult skin)
Specification
adult skin
Permeabilization
Yes - Tween-20
Fixative
Paraformaldehyde
Read More
abreview image
Username

Mr. Heiko Locher

Verified customer

提交于 Sep 20 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MelanA antibody [DT101 + BC199]

 Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lymph node)
Specification
lymph node
Fixative
Acetone
Antigen retrieval step
None
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Read More
abreview image
Username

Abcam user community

Verified customer

提交于 May 01 2009

Hello, I wondered if you could help me please. I am looking to stain human biopsy tissue sections taken from melanoma tumours. I am interested in tumour antigens gp100, MART-1 and tyrosinase. Do you have any antibodies to these tumour antigens suitabl...

Read More

I suggest that you conduct a search through the Abcam Website. I also recommend that you should click on the links “Product Reviews” and “Technical Enquiries” both accessible via the green menu bar on the datasheet. Here you will find customer reviews...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

注册