Anti-Mel18抗体(ab77255)

概述

  • 产品名称Anti-Mel18抗体
    参阅全部 Mel18 一抗
  • 描述
    小鼠单克隆抗体to Mel18
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Chimpanzee, Macaque Monkey
  • 免疫原

    Recombinant fragment: LTLATVPTPS EGTNTSGASE CESVSDKAPS PATLPATSSS LPSPATPSHG SPSSHGPPA, corresponding to amino acids 236-294 of Human Mel18 with a tag of 26kDa

  • 阳性对照
    • Human liver lysate

性能

应用

Our Abpromise guarantee covers the use of ab77255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa).
ELISA Use a concentration of 10 µg/ml. Detection limit is 3ng/ml as a capture antibody when used against the immunogen.

靶标

  • 功能Transcriptional repressor. Binds specifically to the DNA sequence 5'-GACTNGACT-3'. Has tumor suppressor activity. May play a role in control of cell proliferation and/or neural cell development. Regulates proliferation of early T progenitor cells by maintaining expression of HES1. Also plays a role in antero-posterior specification of the axial skeleton and negative regulation of the self-renewal activity of hematopoietic stem cells. Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility.
  • 组织特异性Detected in all tissues examined with high expression found in placenta lung and kidney and low expression, in liver, pancreas and skeletal muscle.
  • 序列相似性Contains 1 RING-type zinc finger.
  • 翻译后修饰Phosphorylated. Homodimer formation is regulated by phosphorylation state with unphosphorylated protein forming homodimers.
  • 细胞定位Nucleus.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DNA binding protein Mel 18 antibody
    • DNA-binding protein Mel-18 antibody
    • Mel 18 antibody
    • MGC10545 antibody
    • PCGF 2 antibody
    • PCGF2 antibody
    • PCGF2_HUMAN antibody
    • Polycomb group ring finger 2 antibody
    • Polycomb group RING finger protein 2 antibody
    • RING finger protein 110 antibody
    • RNF110 antibody
    • Zinc finger protein 144 antibody
    • ZNF 144 antibody
    • ZNF144 antibody
    see all

Anti-Mel18 antibody 图像

  • Anti-Mel18 antibody (ab77255) at 5 µg/ml + immunogen at 0.2 µg/ml

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 38 kDa
    Observed band size : 33 kDa (why is the actual band size different from the predicted?)
  • Anti-Mel18 antibody (ab77255) at 5 µg/ml + Human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 38 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • Detection limit for ab77255 is 3ng/ml as a capture antibody when used against the recombinant immunogen.

Anti-Mel18 antibody (ab77255)参考文献

ab77255 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Human Cell lysate - whole cell (NT2)
Negative control Intergenic region on chromosome 11.
Specification NT2
Detection step Real-time PCR
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% Formaldehyde
Positive control CCND2 and ALX4 genes.
Username

tatiana kan

Verified customer

提交于 Oct 17 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"