概述

  • 产品名称Anti-MEKK3抗体[ep600y]
    参阅全部 MEKK3 一抗
  • 描述
    兔单克隆抗体[ep600y] to MEKK3
  • 经测试应用适用于: ICC/IF, IHC-P, WB, Flow Cyt, IPmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Synthetic peptide corresponding to C-terminal (Human)

  • 阳性对照
    • HeLa cells, ovary carcinoma
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

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应用

Our Abpromise guarantee covers the use of ab40756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/100 - 1/250.
IHC-P Use at an assay dependent concentration.
WB 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 71 kDa).
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/20.

靶标

  • 功能Component of a protein kinase signal transduction cascade. Mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators.
  • 序列相似性Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 OPR domain.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • M3K3_HUMAN antibody
    • MAP/ERK kinase kinase 3 antibody
    • MAP3K3 antibody
    • MAPK/ERK kinase kinase 3 antibody
    • MAPKKK3 antibody
    • MEK kinase 3 antibody
    • MEKK 3 antibody
    • MEKK3 antibody
    • Mitogen activated protein kinase kinase kinase 3 antibody
    • Mitogen-activated protein kinase kinase kinase 3 antibody
    see all

Anti-MEKK3 antibody [ep600y] 图像

  • Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution + HeLa cell lysate at 10 µg

    Predicted band size : 71 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1:100
  • Immunofluorescent staining of HeLa cells using ab40756 diluted 1:100

Anti-MEKK3 antibody [ep600y] (ab40756)参考文献

This product has been referenced in:

See all 5 Publications for this product

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